老年性白内障房水中抗氧化剂和自由基的种类

IF 0.2 4区医学 Q4 MEDICINE, GENERAL & INTERNAL

Srpski arhiv za celokupno lekarstvo Pub Date : 2023-01-01 DOI:10.2298/sarh230619092b

Stefan Brunet, Nikola Babic, Sofija Davidovic, Aleksandar Miljkovic, Nikola Knezi, Vladimir Canadanovic

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引用次数: 0

摘要

介绍/目标。年龄相关性白内障是世界范围内视力损害的重要原因。氧化损伤和自由基的作用在白内障的发病过程中被认为是重要的。本研究的目的是根据年龄和白内障成熟度评估房水的抗氧化能力和氧化应激。方法。对55例老年性白内障进行临床及生化研究。根据白内障成熟程度将患者分为早期(皮质组18例，核N组20例)和成熟(M组17例)。为了评估年龄对患者的影响，将每组患者分为:I组(65-69岁)和II组(70 ?年)。采用还原功率法(RP)和分光光度法测定了房水的抗氧化活性。用电子自旋共振光谱法检测羟基和抗坏血酸自由基浓度的变化。结果。RP和GPx活性均显著降低(p <0.001)， N组与C组相比，M组与N组相比，羟基浓度(29.45 ?C组为1.01%，38.12 ?N组1.29%，74.14 ?M组(2.52%)和抗坏血酸自由基(26.12 ?C组为0.89%，41.15%;N组1.39%，N组83.56 ?M组(2.84%)显著升高(p <0.05)与年龄相关性白内障的进展有关。显著负相关(r = -0.759, p <羟基自由基浓度与GPx含量之间的关系为0.05)。结论。我们的研究证明，房水氧化应激水平在衰老和白内障发展过程中受到显著影响，所获得的数据支持了房水抗氧化能力下降的假设，在衰老过程中，随着白内障的成熟，房水的抗氧化能力随着活性羟基自由基浓度的增加而下降。

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Antioxidant and free radicals species in the aqueous humor of patients with age-related cataract

Introduction/Objective. Age-related cataract is a significant cause of visual impairment worldwide. Oxidative damage and the effects of free radical species are considered important in the etiopathogenesis of cataracts. The aim of this study was to evaluate the antioxidative capacity and oxidative stress in the aqueous humor according to age and cataracts maturity. Methods. Clinical and biochemical researches were carried out in 55 patients with age-related cataract. According to the cataract maturity patients were classified into incipient (cortical-group C, 18 pts, nuclear-group N, 20 pts) and mature (group M, 17 pts). In order to evaluate the impact of age patients within each group were divided in: Group I (65-69 years) and Group II (70 ? years). The antioxidant activity of aqueous humor was measured by the reduction power (RP) method and the activity of glutathione peroxidase (GPx) spectrophotometrically. Changes in the concentrations of hydroxyl and ascorbyl radicals were detected by electron spin resonance spectroscopy. Results. Both RP and GPx activitiy were significantly (p < 0.001) reduced in group N compared to group C and in group M compared to group N. Concentrations of hydroxyl (29.45 ? 1.01% in group C, 38.12 ? 1.29% in group N and 74.14 ? 2.52% in group M) and ascorbyl radicals (26.12 ? 0.89% in group C, 41.15 ? 1.39% in group N and 83.56 ? 2.84% in group M) increased significantly (p < 0.05) with progression of age-related cataract. Significant negative correlation (r = -0.759, p < 0.05) was determined between concentrations of hydroxyl radicals and content of GPx. Conclusion. Our research proved that the level of oxidative stress in the aqueous humor is significantly affected during aging and cataract progression, The obtained data support the hypothesis that during aging, depending on the maturity of the cataract, the antioxidant capacity in the aqueous humor decreases due to an increase in the concentration of reactive hydroxyl radicals.

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来源期刊

Srpski arhiv za celokupno lekarstvo MEDICINE, GENERAL & INTERNAL-

CiteScore

0.40

自引率

50.00%

发文量

104

审稿时长

4-8 weeks

期刊介绍： Srpski Arhiv Za Celokupno Lekarstvo (Serbian Archives of Medicine) is the Journal of the Serbian Medical Society, founded in 1872, which publishes articles by the members of the Serbian Medical Society, subscribers, as well as members of other associations of medical and related fields. The Journal publishes: original articles, communications, case reports, review articles, current topics, articles of history of medicine, articles for practitioners, articles related to the language of medicine, articles on medical ethics (clinical ethics, publication ethics, regulatory standards in medicine), congress and scientific meeting reports, professional news, book reviews, texts for "In memory of...", i.e. In memoriam and Promemoria columns, as well as comments and letters to the Editorial Board. All manuscripts under consideration in the Serbian Archives of Medicine may not be offered or be under consideration for publication elsewhere. Articles must not have been published elsewhere (in part or in full).