青光眼角鲨烯在实验动物模型中的抗炎作用

IF 0.7 Q4 PHARMACOLOGY & PHARMACY

Pharmacognosy Research Pub Date : 2023-11-08 DOI:10.5530/pres.15.4.069

Svenia Periyapurath Jose, Sandya Sukumaran, Ratheesh Mohanan, Sangeeth Saji, Aditya Asish, Sajeev Martin George

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引用次数: 0

摘要

出版日期:2023年10月类型:原创文章作者:Svenia Periyapurath Jose, Sandya Sukumaran, Ratheesh Mohanan, Sangeeth Saji, Aditya Asish, Sajeev Martin George作者单位:Svenia Periyapurath Jose1, Sandya Sukumaran1，*， Ratheesh Mohanan2, Sangeeth Saji2, Aditya Asish1, Sajeev Martin George3 1印度科学研究所无机物理化学系，班加罗尔，Karnataka，印度。2圣托马斯学院生物化学系，印度喀拉拉邦帕莱3印度喀拉拉邦帕莱圣托马斯学院化学系摘要:背景:科学研究最活跃的领域是鉴定生物活性化合物并确定其对慢性疾病的潜在健康作用。副作用小的多靶点化合物已被证明是潜在的治疗药物。目的:观察角鲨烯(一种从青光眼中提取的三萜)对卡拉胶引起的急性炎症的抗炎作用。材料与方法:从青光眼中分离得到角鲨烯(Squalene, SQ)，并用红外光谱(FT-IR)和核磁共振(NMR)对其进行了表征。实验动物分为5组，ⅰ组为对照组(生理盐水中加入0.5% DMSO)，ⅱ组给予卡拉胶(Carr)，ⅲ组给予SQ和Carr，ⅳ组给予双氯芬酸和Carr。1小时后给予SQ和双氯芬酸(20mg/kg) (i.p)。右后爪足底下组织注射卡拉胶悬浮液。给予卡拉胶3小时后测定足跖水肿。处死大鼠，检测TNF-α、IL-6 mRNA表达、PGE-2、TBARS水平、COX-2、SOD、过氧化氢酶、GPx、MPO活性及亚硝酸盐水平。结果:SQ在5.0 mg/kg体重的剂量被发现是最大的水肿抑制的最小剂量。血清TNF-α、IL-6、PGE-2、NO、COX-2水平及TBARS、MPO水平均显著降低(p < 0.05)。SOD、过氧化氢酶、GPx等抗氧化指标显著升高(p < 0.05)。结论:提示SQ具有抗炎作用及其多靶点作用机制，对多种炎症性疾病具有潜在的治疗作用。关键词:抗氧化剂，炎症，MPO, PGE2, SqualeneView

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Anti-inflammatory Effect of Squalene Isolated from Simarouba glauca in Experimental Animal Model

Pharmacognosy Research,2023,15,4,658-666.DOI:10.5530/pres.15.4.069Published:October 2023Type:Original ArticleAuthors:Svenia Periyapurath Jose, Sandya Sukumaran, Ratheesh Mohanan, Sangeeth Saji, Aditya Asish, and Sajeev Martin George Author(s) affiliations:Svenia Periyapurath Jose1, Sandya Sukumaran1,*, Ratheesh Mohanan2, Sangeeth Saji2, Aditya Asish1, Sajeev Martin George3 1Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore, Karnataka, INDIA. 2Department of Biochemistry, St. Thomas College, Palai, Kerala, INDIA. 3Department of Chemistry, St. Thomas College, Palai, Kerala, INDIA. Abstract:Background: Most dynamic area of scientific investigation is to identify the bioactive compounds and establish their potential health effects against chronic diseases. Multi-targeted compounds with fewer side effects has shown to be potential therapeutic agents. Objectives: To evaluate the anti-inflammatory effect of isolated Squalene (SQ), a triterpenoid from Simarouba glauca in carrageenan-induced acute inflammation. Materials and Methods: Squalene (SQ), a triterpenoid fraction was isolated from Simarouba glauca and characterized by FT-IR and NMR. Experimental animals were categorized into five groups, group I was control (0.5% DMSO in normal saline), group II received Carrageenan (Carr), group III received SQ and Carr, whereas group IV received diclofenac and Carr. After 1 hr, SQ and diclofenac (20mg/kg) were administered (i.p.). Carrageenan suspension was injected into the sub-plantar tissue of the right hind paw. Paw edema was determined 3 hours post-carrageenan administration. Rats were sacrificed and mRNA expression of TNF-α and IL-6, levels of PGE-2 and TBARS, activities of COX-2, SOD, catalase, GPx, MPO, and the level of nitrite were measured. Results: SQ at a dose of 5.0 mg/kg body weight was found to be the minimal dose for maximum edema inhibition. Serum levels of TNF-α, IL-6, PGE-2, NO, COX-2 and levels of TBARS and MPO were significantly reduced (p < 0.05). Antioxidant markers such as SOD, Catalse and GPx were increased significantly (p < 0.05). Conclusion: These results suggest the anti-inflammatory properties of SQ and its multi-targeted mechanism of action, meriting its potential therapeutic efficacy in various inflammatory diseases. Keywords:Antioxidants, Inflammation, MPO, PGE2, SqualeneView:PDF (520.4 KB)

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Pharmacognosy Research PHARMACOLOGY & PHARMACY-

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期刊介绍： Pharmacognosy Research [ISSN: Print -0976-4836, Online - 0974-8490] [http://www.phcogres.com], Quarterly a publication of Phcog.Net is published by Wolters Kluwer - Medknow Publications. It provides peer-reviewed original research articles from the field of Natural Products. The journal serves an international audience of scientists and researchers in a variety of research and academia by quickly disseminating research findings related to Medicinal Plants and Natural Products. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submissions of original contributions in all areas of pharmacognosy are welcome.