不同LED品质处理含笑幼苗生长及转录组学分析

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.32604/phyton.2023.030664

Zhaoli Chen, Ying Liu, Bingshan Zeng, Qingbin Jiang, Shengkun Wang, Xiangyang Li

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引用次数: 0

摘要

含笑(Michelia macclurei Dandy)是中国南方广泛种植的重要树种，具有重要的经济和实用价值。在组织培养过程中，光质量影响植株的生长发育。然而，不同光质条件下麦绿霉的生长特性和分子调控尚不清楚。本研究研究了白光、蓝光和红光等不同发光二极管(LED)品质对马尾松幼苗形态、叶绿素含量和转录组学的影响。结果表明:蓝光显著提高了株高(21.29%)和叶片数(18.65%)，而红光显著降低了株高(7.53%)和叶片数(16.49%);与白光相比，蓝光和红光质量显著降低了植株叶绿素含量和黄化率。与白光相比，蓝光对生根率(64.28%)、根数(72.72%)和根长(75.86%)均有不利影响。相反，红光对生根率(24.99%)、根数(109.58%)和根长(72.72%)均有促进作用。转录组分析鉴定出蓝光与白光(BL-vs-WL)、红光与白光(RL-vs-WL)、红光与蓝光(RL-vs-BL)三组中54个差异表达基因(DEGs)。具体来说，在三组中分别鉴定出21、7和41个deg。RL-vs-WL组和BL-vs-WL组中发现的deg参与了植物激素信号转导、氮代谢和苯丙素生物合成等途径，表明麦草通过调节基因表达来适应光质变化。总之，本研究为了解不同光质条件下黄颡鱼幼苗的分子和形态响应提供了有价值的见解。

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Growth and Transcriptomics Analysis of Michelia macclurei Dandy Plantlets with Different LED Quality Treatments

Michelia macclurei Dandy is a significant tree species that has extensive cultivation for forestry and horticulture purposes in southern China, owing to its economic and practical importance. Light quality influences plantlet growth and development during tissue culture. However, the growth characteristic and molecular regulation of M. macclurei under different light quality conditions are not well understood yet. In this study, we investigated the morphological, chlorophyll content, and transcriptomic responses of M. macclurei plantlets under different light-emitting diode (LED) qualities, including white, blue, and red light. The results showed that blue light significantly increased plant height (21.29%) and leaf number (18.65%), while red light decreased plant height and leaf number by 7.53% and 16.49%, respectively. In addition, the plantlets’ chlorophyll content and etiolation rate were significantly reduced by blue and red light quality compared to white light. Compared to white light, blue light had a negative effect, leading to decreased rooting rate (64.28%), root number (72.72%), and root length (75.86%). Conversely, red light had a positive effect, resulting in increased rooting rate (24.99%), root number (109.58%), and root length (72.72%). Transcriptome analysis identified 54 differentially expressed genes (DEGs) in three groups that consisted of blue light vs. white light (BL-vs-WL), red light vs. white light (RL-vs-WL), and red light vs. blue light (RL-vs-BL). Specifically, 21, 7, and 41 DEGs were identified in the three groups, respectively. The DEGs found in the RL-vs-WL and BL-vs-WL groups were involved in plant hormone signaling, nitrogen metabolism, and phenylpropanoid biosynthesis pathways, which suggests that M. macclurei plantlets adapt to the changes of light quality via modulating gene expression. Overall, our study provides valuable insights for understanding the molecular and morphological responses of M. macclurei plantlets under different light qualities.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.