海绵相关芽孢杆菌作为生物防治剂对感染鲶鱼几种细菌的抑制潜力

Q3 Environmental Science
Della Indah Medani
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引用次数: 0

摘要

鲶鱼是印度尼西亚消费和生产水平较高的淡水养殖商品之一。病原菌可在鲶鱼养殖活动中引起疾病暴发。几种致病菌可引起鲶鱼患病,导致大量死亡。这可能导致淡水渔业产品,特别是鲶鱼的食品质量下降。在养殖系统中,养殖病害的发生会造成严重的经济损失。鲶鱼样本取自鲶鱼养殖场,临床症状为身体外部有红色斑点。在无菌条件下从肾脏和肝脏中分离出细菌。通过菌落形态、革兰氏染色、生化试验、分子试验和斑点扩散试验对分离的芽孢杆菌进行抑菌鉴定。基于16S rRNA基因的鉴定表明,GL1与威氏气单胞菌亲缘性为99.92%,与解淀粉芽孢杆菌亲缘性为100%,GL2和HL2与蜡样芽孢杆菌亲缘性为100%。velenzensis菌株APD10菌株抑菌试验结果显示,对GL2致病菌的抑菌范围在极强抑菌区为22.15 mm,对HL2致病菌的抑菌范围在中等抑菌区为8.5 mm。从海绵葡孢杆菌中分离得到的velezensis可进一步作为鲶鱼致病菌蜡样芽孢杆菌的生物防治剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Potential of Sponge-Associated Bacillus spp. as A Biocontrol Agent to Inhibit Several Bacteria from Infected Catfish (Clarias gariepinus Burch)
Catfish is one of the freshwater aquaculture commodities with a high level of consumption and production in Indonesia. Disease outbreaks could occur in catfish farming activities caused by pathogenic bacteria. Several species of pathogenic bacteria can cause disease in catfish, resulting in mass death. This can lead to decrease in the food quality of freshwater fishery products, especially catfish. In cultured system, aquaculture occurrence of diseases can cause severe financial losses. Catfish samples were obtained from catfish farming with clinical symptoms of reddish spots on the outside of the body. Bacteria were isolated from the kidney and liver under aseptic conditions. These bacteria isolates were identified through their colony morphology, Gram staining, biochemical tests, molecular test, and antibacterial test of Bacillus spp. using spot and disc diffusion test. Identification based on 16S rRNA gene showed that GL1 was 99.92% closely related to Aeromonas widowei, HL1 was 100% closely to Bacillus amyloliquefaciens, and GL2 and HL2 was closely related to Bacillus cereus. The antibacterial test results of APD10 isolates of Bacillus velenzensis species inhibited GL2 pathogenic bacteria with an inhibition zone of 22.15 mm in the very strong inhibition zone and HL2 pathogenic bacteria with an inhibition zone of 8.5 mm in the moderate inhibition zone. Bacillus velezensis was isolated from the sponge Aplysina sp. could be further utilized as a biocontrol agent for the pathogenic bacteria, Bacillus cereus, that infects catfish.
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
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