聚乙二醇缺水诱导绿豆抗旱基因型的离体筛选

IF 0.6 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Chiang Mai Journal of Science Pub Date : 2023-05-31 DOI:10.12982/cmjs.2023.035

Chadapon Chaiyapan, Apinya Khairum, Witsarut Chueakhunthod, Pakhawat Pookhamsak, Ketwalee Siwapithakpong, Piyada A. Tantasawat

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引用次数: 0

摘要

干旱胁迫是影响绿豆(Vigna radiata (L.))等几种经济作物生长和产量的主要制约因素。Wilczek]。体外抗旱性筛选是早期确定抗旱性植物的另一种方法。本研究的目的是对聚乙二醇(PEG) 6000诱导的绿豆基因型进行体外筛选。18个基因型的种子在添加不同浓度PEG 6000(0、8、12、16%)的Murashige和Skoog (MS)液体培养基中萌发。测定发芽率、及时性指数、根长耐受性指数、种子活力指数、苗长耐受性指数、幼苗鲜重耐受性指数和幼苗干重耐受性指数。结果表明，随着PEG浓度的增加，所有基因型的萌发和生长都有所降低。不同基因型对16% PEG的反应不同。有趣的是，K-851、EG-MG-13、VI000411 a - g和CES 44的发芽率、根长、苗长和种子鲜重均高于其他基因型，显示了它们的抗旱潜力。然而，它们的水分胁迫反应需要在温室和/或田间条件下得到证实，然后才能作为亲本用于育种计划，以提高耐旱性。

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In Vitro Selection of Mungbean Genotypes for Drought Tolerance by Polyethylene Glycol Induced Water Defi cit

Drought stress is a major constraint affecting growth and yield of several economic crops including mungbean [Vigna radiata (L.) Wilczek]. In vitro selection for drought tolerance is an alternative method for early determination of drought tolerant plants. The objective of this study was to perform in vitro selection of mungbean genotypes under water stress induced by polyethylene glycol (PEG) 6000. Seeds of 18 genotypes were germinated in Murashige and Skoog (MS) liquid medium supplemented with different concentrations of PEG 6000 (0, 8, 12 and 16%). Germination percentage, promptness index, root length tolerance index, seed vigor index, seedling length tolerance index, seedling fresh weight tolerance index and seedling dry weight tolerance index were measured. The results showed reduced germination and growth in all genotypes with increasing PEG concentrations. Various genotypes responded differentially at 16% PEG. Interestingly, K-851, EG-MG-13, VI000411 A-G, and CES 44 showed a higher germination percentage, root length, seedling length and seed fresh weight than other genotypes, demonstrating their drought tolerance potential. However, their water stress responses need to be confi rmed under greenhouse and/or fi eld conditions before they can be used as parents in breeding programs to improve drought tolerance.

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来源期刊

Chiang Mai Journal of Science MULTIDISCIPLINARY SCIENCES-

CiteScore

1.00

自引率

25.00%

发文量

103

审稿时长

3 months

期刊介绍： The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.