{"title":"玉米群体遗传多样性的分子标记分析","authors":"Braulio Torres-Morales, Mario Rocandio-Rodríguez, Amalio Santacruz-Varela, Leobigildo Córdova-Téllez, Bulmaro Coutiño Estrada, Higinio López Sánchez","doi":"10.4081/ija.2023.2206","DOIUrl":null,"url":null,"abstract":"Seventy-three maize populations were characterized to estimate the genetic distribution and structure of 8 maize races from the state of Chiapas, in addition to a population of the Balsas race of teosinte (Zea mays ssp. parviglumis Iltis & Doebley). A total of 31 microsatellite loci were evaluated in 25 individuals from each population, estimating their genetic diversity and Wright F statistics. The populations were grouped based on principal component and cluster analyses. A total of 787 alleles were counted, with an average of 25.4 alleles per locus and 91.8% polymorphic loci. Likewise, in the studied populations, 294 exclusive alleles were detected with low frequency, representing 37% of the total alleles. The populations from Zapalote Grande and Tepecintle races were the most differentiated, forming separate, better-defined groups, while the populations from Comiteco, Otolón, and Negro de Chimaltenango races tended to group, showing a relatively scattered allocation within the races. The FST statistic (differentiation index) was 0.197, indicating that 80.3% of the genetic variation was found among individuals within the accessions, which suggests that, under the current status of Chiapas maize populations, it would be more efficient to apply intra-population recurrent selection than hybridization breeding approaches.","PeriodicalId":14618,"journal":{"name":"Italian Journal of Agronomy","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic diversity characterization of maize populations using molecular markers\",\"authors\":\"Braulio Torres-Morales, Mario Rocandio-Rodríguez, Amalio Santacruz-Varela, Leobigildo Córdova-Téllez, Bulmaro Coutiño Estrada, Higinio López Sánchez\",\"doi\":\"10.4081/ija.2023.2206\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Seventy-three maize populations were characterized to estimate the genetic distribution and structure of 8 maize races from the state of Chiapas, in addition to a population of the Balsas race of teosinte (Zea mays ssp. parviglumis Iltis & Doebley). A total of 31 microsatellite loci were evaluated in 25 individuals from each population, estimating their genetic diversity and Wright F statistics. The populations were grouped based on principal component and cluster analyses. A total of 787 alleles were counted, with an average of 25.4 alleles per locus and 91.8% polymorphic loci. Likewise, in the studied populations, 294 exclusive alleles were detected with low frequency, representing 37% of the total alleles. The populations from Zapalote Grande and Tepecintle races were the most differentiated, forming separate, better-defined groups, while the populations from Comiteco, Otolón, and Negro de Chimaltenango races tended to group, showing a relatively scattered allocation within the races. The FST statistic (differentiation index) was 0.197, indicating that 80.3% of the genetic variation was found among individuals within the accessions, which suggests that, under the current status of Chiapas maize populations, it would be more efficient to apply intra-population recurrent selection than hybridization breeding approaches.\",\"PeriodicalId\":14618,\"journal\":{\"name\":\"Italian Journal of Agronomy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2023-11-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Italian Journal of Agronomy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4081/ija.2023.2206\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Italian Journal of Agronomy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4081/ija.2023.2206","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
摘要
对来自美国恰帕斯州的8个玉米小种和1个大刍草(Zea mays ssp)巴尔萨斯小种的73个玉米群体进行了遗传分布和结构分析。细小肠炎& &;Doebley)。对每个群体25个个体的31个微卫星位点进行了遗传多样性和Wright F统计分析。根据主成分分析和聚类分析对人群进行分组。共检测到787个等位基因,平均每个位点有25.4个等位基因,多态性位点占91.8%。同样,在所研究的人群中,检测到294个低频率的排他性等位基因,占总等位基因的37%。来自Zapalote Grande和Tepecintle种族的种群分化程度最高,形成了独立的、更明确的群体,而来自Comiteco、Otolón和Negro de Chimaltenango种族的种群倾向于群体,在种族内表现出相对分散的分布。FST统计值(分化指数)为0.197,表明80.3%的遗传变异存在于个体间,表明在恰帕斯玉米群体现状下,群体内循环选择比杂交育种更有效。
Genetic diversity characterization of maize populations using molecular markers
Seventy-three maize populations were characterized to estimate the genetic distribution and structure of 8 maize races from the state of Chiapas, in addition to a population of the Balsas race of teosinte (Zea mays ssp. parviglumis Iltis & Doebley). A total of 31 microsatellite loci were evaluated in 25 individuals from each population, estimating their genetic diversity and Wright F statistics. The populations were grouped based on principal component and cluster analyses. A total of 787 alleles were counted, with an average of 25.4 alleles per locus and 91.8% polymorphic loci. Likewise, in the studied populations, 294 exclusive alleles were detected with low frequency, representing 37% of the total alleles. The populations from Zapalote Grande and Tepecintle races were the most differentiated, forming separate, better-defined groups, while the populations from Comiteco, Otolón, and Negro de Chimaltenango races tended to group, showing a relatively scattered allocation within the races. The FST statistic (differentiation index) was 0.197, indicating that 80.3% of the genetic variation was found among individuals within the accessions, which suggests that, under the current status of Chiapas maize populations, it would be more efficient to apply intra-population recurrent selection than hybridization breeding approaches.
期刊介绍:
The Italian Journal of Agronomy (IJA) is the official journal of the Italian Society for Agronomy. It publishes quarterly original articles and reviews reporting experimental and theoretical contributions to agronomy and crop science, with main emphasis on original articles from Italy and countries having similar agricultural conditions. The journal deals with all aspects of Agricultural and Environmental Sciences, the interactions between cropping systems and sustainable development. Multidisciplinary articles that bridge agronomy with ecology, environmental and social sciences are also welcome.