FRZB基因通过Wnt/β-catenin信号通路调控家兔毛囊发育

IF 0.8 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

World Rabbit Science Pub Date : 2023-09-28 DOI:10.4995/wrs.2023.18171

Fan Wang, Xiyu Zhang, Yingying Dai, Bohao Zhao, Xinsheng Wu, Yang Chen

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引用次数: 0

摘要

为了探讨FRZB基因通过调控Wnt/β-catenin信号通路在毛囊发育中的作用机制，我们选择安哥拉兔采集背部皮肤样本进行实验。通过细胞培养和转染、细胞凋亡和增殖试验以及TOP/FOP Flash Wnt报告系统方法了解其作用机制。结果表明，FRZB基因在兔真皮乳头细胞中的干扰和过表达表明，过表达可抑制SFRP2、BMP4和WNT2基因的表达(P<0.05)。相反，Wnt信号通路相关基因LEF1、CCND1、DKK1和TCF7的表达显著上调(P <0.05)。进一步检测荧光素酶报告系统TOP/FOP发现pcDNA3.1-FRZB抑制Wnt活性。发现PcDNA3.1-FRZB促进DP细胞凋亡水平，而si-FRZB抑制DP细胞增殖。因此，FRZB可能通过调控Wnt/β-catenin信号通路抑制长毛兔毛囊发育。

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The FRZB gene regulates hair follicle development in rabbits via the Wnt/β-catenin signaling pathway

To explore the mechanism of the FRZB gene in hair follicle development by regulating the Wnt/β-catenin signalling pathway, Angora rabbits were selected to collect back skin samples for the experiment. The action mechanism is understood by cell culture and transfection, apoptosis and proliferation assays and TOP/FOP Flash Wnt Reporting System methods. The results showed that the interference and overexpression of the FRZB gene in rabbit dermal papilla cells indicated that overexpression could inhibit the expression of SFRP2, BMP4, and WNT2 genes (P<0.05). On the contrary, the expression of Wnt signalling pathway-related genes LEF1, CCND1, DKK1, and TCF7 was significantly up-regulated (P < 0.05). Further examination of the luciferase reporter system TOP/FOP revealed that pcDNA3.1-FRZB inhibits Wnt activity. PcDNA3.1-FRZB was found to promote the level of apoptosis in DP cells, whereas si-FRZB inhibited DP cell proliferation. Therefore, it is concluded that FRZB inhibits hair follicle development in long-haired rabbits by regulating the Wnt/β-catenin signalling pathway.

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来源期刊

World Rabbit Science 农林科学-奶制品与动物科学

CiteScore

1.70

自引率

25.00%

发文量

审稿时长

>36 weeks

期刊介绍： World Rabbit Science is the official journal of the World Rabbit Science Association (WRSA). One of the main objectives of the WRSA is to encourage communication and collaboration among individuals and organisations associated with rabbit production and rabbit science in general. Subject areas include breeding, genetics, production, management, environment, health, nutrition, physiology, reproduction, behaviour, welfare, immunology, molecular biology, metabolism, processing and products. World Rabbit Science is the only international peer-reviewed journal included in the ISI Thomson list dedicated to publish original research in the field of rabbit science. Papers or reviews of the literature submitted to World Rabbit Science must not have been published previously in an international refereed scientific journal. Previous presentations at a scientific meeting, field day reports or similar documents can be published in World Rabbit Science, but they will be also subjected to the peer-review process. World Rabbit Science will publish papers of international relevance including original research articles, descriptions of novel techniques, contemporaryreviews and meta-analyses. Short communications will only accepted in special cases where, in the Editor''s judgement, the contents are exceptionally exciting, novel or timely. Proceedings of rabbit scientific meetings and conference reports will be considered for special issues. World Rabbit Science is published in English four times a year in a single volume. Authors may publish in World Rabbit Science regardless of the membership in the World Rabbit Science Association, even if joining the WRSA is encouraged. Views expressed in papers published in World Rabbit Science represent the opinion of the author(s) and do not necessarily reflect the official policy of the WRSA or the Editor-in-Chief.