结核单叶树生物碱和多酚提取物的特性及抗菌活性研究

Djamila DJOUAHRA FAHEM, Souhila BENSMAIL, Razika BOUTELDJA, Messad SARA, Fatiha FERHOUM, Nassima BOURFIS, Fatma ACHEUK, Fethia FAZOUANE
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引用次数: 0

摘要

小叶蕨是一种药用植物,属于芸香科,以其多种治疗特性而闻名。本研究旨在对该植物的生物碱和多酚提取物进行表征,并评价其抗菌活性。本研究采用浸渍法从该植物中提取多酚和生物碱。采用Folin Ciacalteu法测定总酚含量,采用薄层色谱法对两种提取物进行定性表征。在固体培养基上采用圆盘扩散法测定两种提取物的抑菌活性,并用琼脂稀释法测定两种提取物对敏感菌的最低抑菌浓度(MIC)。结果表明,多酚和生物碱提取物的提取率分别为8.39%和0.37%,而多酚提取物的总酚含量估计为74.45 mg GAE/g。薄层色谱分析选择甲苯-醋酸酯-乙醇-浓氨(40:4:8:3,v/v)体系分离结核分枝杆菌生物碱,选择乙酸乙酯-甲醇-水(100:13:5:10,v/v/)体系分离结核分枝杆菌多酚。同样的分析在多酚提取物中检测到槲皮素、儿茶素和芦丁的痕迹。我们的研究结果表明,革兰氏阳性菌株如金黄色葡萄球菌ATCC 25923和枯草芽孢杆菌ATCC 6633具有良好的抗菌活性,对铜绿假单胞菌ATCC 27953具有中等活性,生物碱的mic范围为0.625 ~ 10 mg/mL,多酚的mic范围为5 ~ 20 mg/mL。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization and antibacterial activity of alkaloids and polyphenols extracts from Haplophyllum tuberculatum (forssk.)
Haplophyllum tuberculatum is a medicinal plant belonging to the Rutaceae family, is renowned for its various therapeutic properties. This study aims to characterize the alkaloids and polyphenol extracts from this plant and assess their antibacterial activity. Herein, the extraction of polyphenols and alkaloids from this plant was performed by the maceration method. Folin Ciacalteu’s method was used to estimate the total phenolic content, and the qualitative characterization of the two extracts was performed by thin-layer chromatography. Whilst, the antibacterial activity of the two extracts was tested with the disk diffusion method on a solid medium and the minimal inhibitory concentration (MIC) of susceptible bacteria was determined using the agar dilution method. Our results indicate respective yields of 8.39% in polyphenols and 0.37% in alkaloid extracts, while the total phenolic content was estimated to be 74.45 mg GAE/g of dry matter in polyphenolic extract. Thin-layer chromatography analysis allowed choosing the system toluene-acetate-ethanol-concentrated ammonia (40:4:8:3, v/v) to separate H. tuberculatum alkaloids, and ethyl acetate-methanol-water (100:13.5:10, v/v/) for the separation of its polyphenols. The same analysis detected traces of quercitin, catechin, and rutin in the polyphenolic extract. Our findings demonstrated good antibacterial activity on Gram-positive strains such as Staphylococcus aureus ATCC 25923 and Bacillus subtilis ATCC 6633, and moderate activity on Pseudomonas aeruginosa ATCC 27953, with MICs ranging from 0.625 to 10 mg/mL for alkaloids and from 5 to 20 mg/mL for polyphenols.
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来源期刊
International Journal of Secondary Metabolite
International Journal of Secondary Metabolite Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
1.40
自引率
0.00%
发文量
28
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