尼日利亚拉各斯临床和社区环境中与金黄色葡萄球菌致病性相关的辅助基因调控因子和毒力基因

IF 1.5 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES
Nkechi V. Enwuru, Solayide A. Adesida, Christian A. Enwuru, Udoma E. Mendie
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引用次数: 0

摘要

金黄色葡萄球菌是全球范围内引起严重社区和医院获得性感染的重要病原体。其致病性归因于多种分泌蛋白和细胞表面相关蛋白,这些蛋白由群体感应辅助基因调节器(agr)系统调节。在这项研究中,我们从临床样本和表面健康的个体中调查了金黄色葡萄球菌涉及的毒素基因和agr的存在。用Vitek 2系统对分离株进行了明确的鉴定。我们采用PCR技术筛选了70株临床(CL)和22株社区(C)金黄色葡萄球菌耐甲氧西林(mecA)基因、agr和超级抗原(SAg)(肠毒素和中毒性休克综合征毒素-1)。12株临床分离株被分类为耐甲氧西林金黄色葡萄球菌(MRSA);89株分离株属于4个agr类群(ag1 ~ 4)之一,3株分离株不可分型。在agr组中,agr1最为突出,主要由脓液/伤口分离株组成。甲氧西林敏感金黄色葡萄球菌(MSSA)分布在4个agr组中,MRSA菌株仅局限于agr1和agr3。同样,最常见的肠毒素基因sei在MSSA菌株中比在MRSA菌株中更为普遍,后者以sea为主。在40%的分离株中证实存在两种或两种以上的肠毒素。除ag3外,其余agr组均出现Sea,而在ag1和ag4中未发现sei。在6例MSSA中检测到毒性休克毒素(tst)基因。这些发现表明,MSSA可能比MRSA引起更致命的感染,因为MSSA菌株中毒性基因型的频率增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Accessory gene regulators and virulence genes associated with the pathogenicity of Staphylococcus aureus from clinical and community settings in Lagos, Nigeria
Staphylococcus aureus is a prominent pathogen that causes serious community and hospital-acquired infections globally. Its pathogenicity is attributed to a variety of secreted and cell surface associated proteins that are modulated by the quorum-sensing accessory gene regulator (agr) system. In this study, we investigated the presence of toxin genes and agr involved with S. aureus from clinical samples and apparently healthy individuals. Unequivocal identification of the isolates was obtained with the Vitek 2 system. We screened 70 clinical (CL) and 22 community (C) S. aureus strains for the methicillin resistance (mecA) gene, agr and superantigens (SAg) (enterotoxins and toxic shock syndrome toxin-1) using PCR techniques. A total of 12 clinical isolates were classified as methicillin-resistant S. aureus (MRSA); 89 isolates belonged to one of the four agr groups (agr1-4), and 3 isolates were non-typeable. Of the agr groups, agr1 was the most prominent and mostly consisted of isolates from pus/wounds. The methicillin-susceptible S. aureus (MSSA) isolates were distributed within the four agr groups while MRSA strains were restricted to agr1 and agr3. The most common enterotoxin gene, sei, was likewise more prevalent in MSSA strains than in MRSA strains, where sea predominated. The co-existence of two or more enterotoxins was confirmed in 40% of the isolates. sea occurred through all the agr groups except agr3 and sei was not found in agr1 and agr4. The toxic shock toxin (tst) gene was detected in six MSSA. These findings suggest that MSSA may cause more lethal infections than MRSA because of the increased frequency of toxic genotypes seen in MSSA strains.
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来源期刊
South African Journal of Science
South African Journal of Science 综合性期刊-综合性期刊
CiteScore
3.20
自引率
4.20%
发文量
131
审稿时长
1 months
期刊介绍: The South African Journal of Science is a multidisciplinary journal published bimonthly by the Academy of Science of South Africa. Our mandate is to publish original research with an interdisciplinary or regional focus, which will interest readers from more than one discipline, and to provide a forum for discussion of news and developments in research and higher education. Authors are requested to write their papers and reports in a manner and style that is intelligible to specialists and non-specialists alike. Research contributions, which are peer reviewed, are of three kinds: Review Articles, Research Articles and Research Letters.
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