Serological detection of bovine tuberculosis and John’s disease by Elisa in South Gujarat

Bovine tuberculosis is a chronic bacterial disease of cattle characterized by respiratory signs and production of progressive granulomatous lesions caused by Mycobacterium bovis. Johne’s disease (JD) is a chronic progressive granulomatous enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). A study was conducted from 2019- 2021 for prevalence of bovine tuberculosis and Johne's disease in cattle and buffaloes from organized dairy farms of south Gujarat region, India by ELISA technique. Total 719 bovine serum samples comprising 461 cattle, 41 bull and 217 buffaloes for bovine tuberculosis, while 637 serum samples comprising 380 cattle, 40 bull and 217 buffaloes were screened for prevalence of Johne's disease. Overall prevalence of bovine tuberculosis and Johne’s disease recorded was 6.25% (45 of 719 animals) and 8.63% (55 of 637 animals), (p>0.05) respectively. Animal wise higher prevalence was noted in cow compared to buffaloes for bovine tuberculosis and Johne’s disease. Among cattle higher prevalence was recorded in female animals than male animals for TB and JD. ELISA is considered as rapid, simple and sensitive technique that could is used for screening and detection of the infected cases of TB and JD in bovines.