抗氧化姜黄倍半萜姜黄酮的分离与表征

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
Ririn Suharsanti, Subagus Wahyuono, Puji Astuti, Nunung Yuniarti
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引用次数: 0

摘要

抗氧化化合物是必需的,以阻止氧化链反应的启动和抑制活性氧的形成,这是各种疾病的原因。天然抗氧化剂可以从多种来源分离,其中一种是从铜绿姜黄中分离出来的。本研究旨在从铜绿姜黄根茎中分离鉴定抗氧化化合物。采用2,2-二苯基-1-苦氨酰基(DPPH)自由基清除活性指示剂分离活性化合物。首先将铜绿姜黄根茎粉末材料用70%的乙醇浸泡,得到乙醇提取物(EE)。然后,随着溶剂极性的增加,EE逐渐分馏得到正己烷(HF)、乙酸乙酯(EAF)、乙醇(EF)和不溶性(IF)馏分。EF为活性组分,根据其TLC图谱分为6个组分(EE.a-f)。两个化合物(EF。通过制备薄层色谱法从活性部位(EF.a)中分离得到a1和EF.a2。EF。a2呈白色晶体,具有较好的抗DPPH活性,纯度为96.24% (HPLC)。EF。a2在GC中呈单峰出现(Rt 12,78),分子量为m/z 234。紫外可见光谱在226 nm和260 nm处有2个吸收峰,FT-IR光谱在3364、3314、2905和1653 cm-1处有吸收带。根据光谱数据(1H-,13C-NMR)和与报道数据的比较,EF。a2鉴定为倍半萜,姜黄酮(C15H22O2)。姜黄酮对DPPH自由基的清除IC50为53.24±1.51µg/mL,对ABTS自由基的清除IC50为41.33±3.15µg/mL,对FRAP自由基的清除IC50为37,82±2.02µg/mL。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and Characterization of Curcumenotone, a Sesquiterpene from Curcuma aeruginosa Roxb as Antioxidant
Antioxidant compounds are necessary to block the initiation of oxidation chain reactions and inhibit the formation of ROS which are the cause of various diseases. Natural antioxidants can be isolated from various sources, one of them from Curcuma aeruginosa Roxb Rhizome. This study was aimed to isolate and identify antioxidant compounds from Curcuma aeruginosa Roxb rhizome. To isolate the active compound, the 2,2-diphenyl-1-picrylhidrazyl (DPPH) free radical scavenging activity guide was used. Initially the Curcuma aeruginosa Roxb rhizome powdered material macerated with ethanol 70% to give ethanol extract (EE). The EE was then fractionated gradually with increasing polarity solvents to give n-hexane (HF), ethyl acetate (EAF), ethanol (EF) and insoluble (IF) fractions. The EF was the active fraction, was fractionated into 6 fractions (EE.a-f) based on their TLC picture. Two compounds (EF.a1 and EF.a2) were isolated from active fraction (EF.a) by preparative TLC. The EF.a2 appeared as white crystals demonstrates better activity as anti radical DPPH, having 96.24% purity (HPLC). EF.a2 appeared as a single peak in GC (Rt 12,78), and having molecular weight at m/z 234. There were 2 absorbtion peaks at 226 nm and 260 nm in Uv-Vis spectrophotometer and absorption bands at 3364, 3314, 2905 and 1653 cm-1 on the FT-IR spectrum. Based on spectroscopic data (1H-,13C-NMR) and comparison with reported data, the EF.a2 was identified as sesquiterpene, curcumenotone (C15H22O2). The curcumenotone was shown potent antioxidant in DPPH radical scavenging assay had an IC50 53.24±1.51 µg/mL, ABTS radical scavenging assay had an IC50 41.33±3.15 µg/mL, and FRAP assay had an IC50 37,82±2.02 µg/mL.
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来源期刊
INDONESIAN JOURNAL OF PHARMACY
INDONESIAN JOURNAL OF PHARMACY PHARMACOLOGY & PHARMACY-
CiteScore
1.20
自引率
0.00%
发文量
38
审稿时长
12 weeks
期刊介绍: The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education. The journal includes various fields of pharmaceuticals sciences such as: -Pharmacology and Toxicology -Pharmacokinetics -Community and Clinical Pharmacy -Pharmaceutical Chemistry -Pharmaceutical Biology -Pharmaceutics -Pharmaceutical Technology -Biopharmaceutics -Pharmaceutical Microbiology and Biotechnology -Alternative medicines.
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