{"title":"使用SOLIS的类器官即时体积显微镜","authors":"Florian Ströhl","doi":"10.1093/mictod/qaad064","DOIUrl":null,"url":null,"abstract":"Abstract Advancements in microscopy techniques have revolutionized our ability to explore the intricacies of biological systems, with engineered human heart tissue (EHT) being a particularly challenging target. In this article, we will provide an in-depth look at the award-winning SOLIS technique (scanned oblique light-sheet instant-volume sectioning), a new twist on multifocus fluorescence microscopy. Through its unique capabilities, SOLIS offers a new approach to organoid and tissue imaging, providing unprecedented insights into the cellular architecture of these complex artificial samples. By being able to record optically sectioned volumes during single-camera exposures, SOLIS demonstrates remarkable advantages over traditional multifocus microscopy, underscoring its potential to transform our understanding of developmental biology, disease mechanisms, and potential therapeutic interventions.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":"307 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Instant Volume Microscopy of Organoids with SOLIS\",\"authors\":\"Florian Ströhl\",\"doi\":\"10.1093/mictod/qaad064\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Advancements in microscopy techniques have revolutionized our ability to explore the intricacies of biological systems, with engineered human heart tissue (EHT) being a particularly challenging target. In this article, we will provide an in-depth look at the award-winning SOLIS technique (scanned oblique light-sheet instant-volume sectioning), a new twist on multifocus fluorescence microscopy. Through its unique capabilities, SOLIS offers a new approach to organoid and tissue imaging, providing unprecedented insights into the cellular architecture of these complex artificial samples. By being able to record optically sectioned volumes during single-camera exposures, SOLIS demonstrates remarkable advantages over traditional multifocus microscopy, underscoring its potential to transform our understanding of developmental biology, disease mechanisms, and potential therapeutic interventions.\",\"PeriodicalId\":74194,\"journal\":{\"name\":\"Microscopy today\",\"volume\":\"307 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microscopy today\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1093/mictod/qaad064\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopy today","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/mictod/qaad064","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Abstract Advancements in microscopy techniques have revolutionized our ability to explore the intricacies of biological systems, with engineered human heart tissue (EHT) being a particularly challenging target. In this article, we will provide an in-depth look at the award-winning SOLIS technique (scanned oblique light-sheet instant-volume sectioning), a new twist on multifocus fluorescence microscopy. Through its unique capabilities, SOLIS offers a new approach to organoid and tissue imaging, providing unprecedented insights into the cellular architecture of these complex artificial samples. By being able to record optically sectioned volumes during single-camera exposures, SOLIS demonstrates remarkable advantages over traditional multifocus microscopy, underscoring its potential to transform our understanding of developmental biology, disease mechanisms, and potential therapeutic interventions.