使用SOLIS的类器官即时体积显微镜

Florian Ströhl
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引用次数: 0

摘要

显微镜技术的进步彻底改变了我们探索生物系统复杂性的能力,工程人类心脏组织(EHT)是一个特别具有挑战性的目标。在本文中,我们将深入研究屡获殊荣的SOLIS技术(扫描斜光片即时体积切片),这是多焦点荧光显微镜的一种新方法。通过其独特的功能,SOLIS为类器官和组织成像提供了一种新的方法,为这些复杂人工样本的细胞结构提供了前所未有的见解。SOLIS能够在单相机曝光时记录光学切片体积,与传统的多焦点显微镜相比,它具有显著的优势,强调了它改变我们对发育生物学、疾病机制和潜在治疗干预的理解的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Instant Volume Microscopy of Organoids with SOLIS
Abstract Advancements in microscopy techniques have revolutionized our ability to explore the intricacies of biological systems, with engineered human heart tissue (EHT) being a particularly challenging target. In this article, we will provide an in-depth look at the award-winning SOLIS technique (scanned oblique light-sheet instant-volume sectioning), a new twist on multifocus fluorescence microscopy. Through its unique capabilities, SOLIS offers a new approach to organoid and tissue imaging, providing unprecedented insights into the cellular architecture of these complex artificial samples. By being able to record optically sectioned volumes during single-camera exposures, SOLIS demonstrates remarkable advantages over traditional multifocus microscopy, underscoring its potential to transform our understanding of developmental biology, disease mechanisms, and potential therapeutic interventions.
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