利用DNA标记对旱稻抗旱基因qDTY1.1的存在进行估计

P. I. Kostylev, N. N. Vozhzhova, A. V. Aksenov
{"title":"利用DNA标记对旱稻抗旱基因qDTY1.1的存在进行估计","authors":"P. I. Kostylev, N. N. Vozhzhova, A. V. Aksenov","doi":"10.31367/2079-8725-2023-88-5-48-55","DOIUrl":null,"url":null,"abstract":"Drought is the main stress factor when growing plants. The effects of drought on rice plants range from reduced dry matter accumulation to poor distribution of metabolites from stems and leaves into grain, resulting in reduced number of full grains per panicle, reduced grain weight and ultimately yield. Rice varietal diversity includes different drought tolerance genes. The purpose of the current research was to estimate upland rice samples and varieties for drought resistance under field conditions and the presence of the qDTY1.1 gene using the DNA marker RM431. PCR analysis was used to estimate 66 rice lines, hybrids and varieties grown in periodically wetted and constantly flooded fields. As a result of marker analysis, there has been identified the presence of the drought resistance gene qDTY1.1 in twenty-two rice varieties and samples such as ‘An-Yun-Ho’, ‘Volgogradsky’, ‘Stalingradsky’, ‘Volgogradsky x Atlant’, ‘Chan-Chun-Man’, etc. The highest value of the drought resistance index (DRI) was found in carriers of the qDTY1.1 allele, they are Manchurian upland varieties ‘An-Yun-Ho’ (79.4 %), ‘Chan-Chun-Man’ (88.5 %) and other samples developed earlier from crossing the variety ‘Chan-Chun-Man’ with productive varieties of Don selection ‘Razdolny’, ‘Boyarin’, ‘Komandor’, ‘Yuzhanin’, ‘Kuboyar’. The identified samples carrying the qDTY1.1 gene exceeded the other forms on average in terms of productivity under drought conditions by 0.20 t/ha, under the control by 0.21 t/ha, and under IZU by 3.9 %. In the long term of improving rice drought tolerance, it is necessary to identify and exploit other QTLs with large and consistent effects and key regulators of plant stress responses.","PeriodicalId":33209,"journal":{"name":"Zernovoe khoziaistvo Rossii","volume":" 28","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Estimation of upland rice samples for the presence of the drought resistance gene qDTY1.1 using a DNA marker\",\"authors\":\"P. I. Kostylev, N. N. Vozhzhova, A. V. Aksenov\",\"doi\":\"10.31367/2079-8725-2023-88-5-48-55\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Drought is the main stress factor when growing plants. The effects of drought on rice plants range from reduced dry matter accumulation to poor distribution of metabolites from stems and leaves into grain, resulting in reduced number of full grains per panicle, reduced grain weight and ultimately yield. Rice varietal diversity includes different drought tolerance genes. The purpose of the current research was to estimate upland rice samples and varieties for drought resistance under field conditions and the presence of the qDTY1.1 gene using the DNA marker RM431. PCR analysis was used to estimate 66 rice lines, hybrids and varieties grown in periodically wetted and constantly flooded fields. As a result of marker analysis, there has been identified the presence of the drought resistance gene qDTY1.1 in twenty-two rice varieties and samples such as ‘An-Yun-Ho’, ‘Volgogradsky’, ‘Stalingradsky’, ‘Volgogradsky x Atlant’, ‘Chan-Chun-Man’, etc. The highest value of the drought resistance index (DRI) was found in carriers of the qDTY1.1 allele, they are Manchurian upland varieties ‘An-Yun-Ho’ (79.4 %), ‘Chan-Chun-Man’ (88.5 %) and other samples developed earlier from crossing the variety ‘Chan-Chun-Man’ with productive varieties of Don selection ‘Razdolny’, ‘Boyarin’, ‘Komandor’, ‘Yuzhanin’, ‘Kuboyar’. The identified samples carrying the qDTY1.1 gene exceeded the other forms on average in terms of productivity under drought conditions by 0.20 t/ha, under the control by 0.21 t/ha, and under IZU by 3.9 %. In the long term of improving rice drought tolerance, it is necessary to identify and exploit other QTLs with large and consistent effects and key regulators of plant stress responses.\",\"PeriodicalId\":33209,\"journal\":{\"name\":\"Zernovoe khoziaistvo Rossii\",\"volume\":\" 28\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-11-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zernovoe khoziaistvo Rossii\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31367/2079-8725-2023-88-5-48-55\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zernovoe khoziaistvo Rossii","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31367/2079-8725-2023-88-5-48-55","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

干旱是植物生长的主要胁迫因素。干旱对水稻植株的影响主要表现在干物质积累减少,茎叶代谢物向籽粒的分配不佳,导致每穗实粒数减少,籽粒重降低,最终影响产量。水稻品种多样性包括不同的抗旱基因。本研究的目的是利用DNA标记RM431估计旱稻样品和品种在田间条件下的抗旱性和qDTY1.1基因的存在。采用PCR方法对66个生长在周期性湿润和经常淹水田中水稻品系、杂交种和品种进行了估计。通过标记分析,在“安运禾”、“伏尔戈格拉斯基”、“斯大林格勒斯基”、“伏尔戈格拉斯基×亚特兰大”、“湛春满”等22个水稻品种和样品中鉴定出了qDTY1.1抗旱基因。qDTY1.1等位基因载体的抗旱指数(DRI)最高的是东北旱地品种‘安运河’(79.4%)、‘灿春满’(88.5%)和其他较早由‘灿春满’品种与Don选育品种‘Razdolny’、‘Boyarin’、‘Komandor’、‘Yuzhanin’、‘Kuboyar’杂交而成的样品。携带qDTY1.1基因的样品在干旱条件下的平均产量比其他品种高出0.20 t/ha,在对照条件下高出0.21 t/ha,在IZU条件下高出3.9%。在提高水稻抗旱性的长期研究中,有必要鉴定和开发其他具有较大且一致效应的qtl和植物逆境响应的关键调控因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Estimation of upland rice samples for the presence of the drought resistance gene qDTY1.1 using a DNA marker
Drought is the main stress factor when growing plants. The effects of drought on rice plants range from reduced dry matter accumulation to poor distribution of metabolites from stems and leaves into grain, resulting in reduced number of full grains per panicle, reduced grain weight and ultimately yield. Rice varietal diversity includes different drought tolerance genes. The purpose of the current research was to estimate upland rice samples and varieties for drought resistance under field conditions and the presence of the qDTY1.1 gene using the DNA marker RM431. PCR analysis was used to estimate 66 rice lines, hybrids and varieties grown in periodically wetted and constantly flooded fields. As a result of marker analysis, there has been identified the presence of the drought resistance gene qDTY1.1 in twenty-two rice varieties and samples such as ‘An-Yun-Ho’, ‘Volgogradsky’, ‘Stalingradsky’, ‘Volgogradsky x Atlant’, ‘Chan-Chun-Man’, etc. The highest value of the drought resistance index (DRI) was found in carriers of the qDTY1.1 allele, they are Manchurian upland varieties ‘An-Yun-Ho’ (79.4 %), ‘Chan-Chun-Man’ (88.5 %) and other samples developed earlier from crossing the variety ‘Chan-Chun-Man’ with productive varieties of Don selection ‘Razdolny’, ‘Boyarin’, ‘Komandor’, ‘Yuzhanin’, ‘Kuboyar’. The identified samples carrying the qDTY1.1 gene exceeded the other forms on average in terms of productivity under drought conditions by 0.20 t/ha, under the control by 0.21 t/ha, and under IZU by 3.9 %. In the long term of improving rice drought tolerance, it is necessary to identify and exploit other QTLs with large and consistent effects and key regulators of plant stress responses.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
45
审稿时长
8 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信