铜绿假单胞菌临床分离株毒力因子编码基因的分子检测与表达

Q4 Biochemistry, Genetics and Molecular Biology
Alyaa Ghaffar Husain, Bahaa Abdullah Laftaah Alrubaii
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引用次数: 0

摘要

简介和目的:铜绿假单胞菌毒力因子基因不仅参与其致病性,而且还引起细菌对多种抗生素的耐药性,因此越来越受到关注。实验室鉴定携带毒力基因的临床分离株对于限制细菌的传播和降低其致病性至关重要。本研究的目的是研究一种简单、廉价的实时PCR检测方法,以检测特定浓度嗜酸乳杆菌无细胞上清液(CFSs)处理前后铜绿假单胞菌毒力基因的表达水平。材料和方法:在2021年12月至2022年6月期间,对从伊拉克巴格达医院收集的350份临床样本进行了铜绿假单胞菌的检测。采用Kirby-Bauer纸片扩散法对分离得到的铜绿假单胞菌进行了药敏试验。采用逆转录- pcr方法检测铜绿假单胞菌毒力基因。real-time PCR检测嗜酸乳杆菌无细胞上清液处理前后这些基因的表达水平。结果:在350个检测样本中,60个分离株铜绿假单胞菌呈阳性。抗生素敏感性试验显示出高水平的抗生素耐药性,而遗传技术鉴定出在嗜酸乳杆菌上清液影响下表现出可变表达的几种毒力基因的存在。结论:研究结果表明,嗜酸乳杆菌上清液可降低铜绿假单胞菌某些毒力基因的表达,提示嗜酸乳杆菌可作为治疗铜绿假单胞菌感染的一种选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular detection and expression of virulence factor encoding genes of Pseudomonas aeruginosa isolated from clinical samples
Introduction and Aim: Pseudomonas aeruginosa virulence factors genes are a growing concern as they are involved not only in its pathogenicity, but also cause bacterial resistance to multiple classes of antibiotics. Laboratory identification of clinical isolates carrying the virulence genes would be critical in limiting the bacteria's spread and reducing its pathogenicity. The purpose of this study was to investigate a simple and inexpensive real-time PCR test to find the level of expression of Pseudomonas aeruginosa virulence genes before and after treatment with specific concentration of Lactobacillus acidophilus cell-free supernatants (CFSs). Materials and Methods: Between December 2021 and June 2022, 350 clinical samples collected from Baghdad hospitals, Iraq, were tested for the presence of P. aeruginosa. The P. aeruginosa isolated were tested for their antimicrobial susceptibility using the Kirby-Bauer disk diffusion method. P. aeruginosa virulence genes were detected by using the reverse transcription-PCR method. The expression levels of these genes before and after treatment with Lactobacillus acidophilus cell-free supernatants were measured by real-time PCR. Results: Out of 350 samples tested, 60 isolates were positive for the presence of P. aeruginosa. Antibiotic susceptibility tests revealed a high level of antibiotic resistance, while genetic techniques identified the presence of several virulence genes that exhibited variable expression under the influence of Lactobacillus acidophilus supernatants. Conclusion: The study findings showed that L. acidophilus supernatants had an effect on reducing the expression of certain virulence genes of P. aeruginosa, implying that L. acidophilus could be used as an option in treating P. aeruginosa infection.
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来源期刊
Biomedicine (India)
Biomedicine (India) Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
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