田间条件下F2群体抗亚洲大豆锈病的Rpp基因

IF 1.2 4区农林科学 Q2 Agricultural and Biological Sciences

Bragantia Pub Date : 2023-01-01 DOI:10.1590/1678-4499.20230099

Daniela Meira, Vinícius de Bitencourt Bez Batti, Leomar Guilherme Woyann, Anderson Simionato Milioli, Antonio Henrique Bozi, Eduardo Beche, Maiara Cecilia Panho, Fabiana Barrinouevo, Laura Alexandra Madella, Gaspar Malone, Salvador Lima Brito Júnior, Taciane Finatto, Giovani Benin

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引用次数: 0

摘要

本研究的目的是利用为Rpp1 - Rpp5开发的KASP标记确定抗性来源，并在F2群体中进行抗性筛选。10 F2大豆(甘氨酸max (L.))对易锈源(55I57RSF IPRO、63I64RSF IPRO)和抗锈源(PI 200492、PI 594538A、PI 587880A、PI 594723、PI 230970、PI 506764、PI 459025A和PI 200487)杂交得到的种群进行了评价。所有F2植株分别在田间条件下进行ASR表型反应评价，并根据产孢量进行分类。KASP标记是根据SoyBase上可用的Rpp基因相关分析开发的。根据PI 200492在图谱位置上的细微差异和不同的表型疾病反应，我们推测PI 594723携带一个抗性基因Rpp1-b。Rpp1-b基因定位在12.4 cM区域的Chr 18上。与携带Rpp1的品系(PI 200492)相比，携带Rpp1-b的品系(PI 594723、PI 587880A和594538A)对ASR的抗性较强。26个KASP标记与ASR抗性显著相关(P < 0.01)。其中，M1、M5和M6 (Rpp1)、M13和M14 (Rpp2)、M16、M17和M20 (Rpp3)、M25和M26 (Rpp4)、M27和M28 (Rpp5)具有应用于标记辅助选择的潜力。

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Rpp genes conferring resistance to Asian soybean rust in F2 population in the field conditions

In this study, the aim of this study was to identify the source of resistance using KASP markers developed for Rpp1 – Rpp5 and screening for resistance in field trials in F2 populations. Ten F2 soybean (Glycine max (L.) Merrill) populations derived from crosses between rust-susceptible (55I57RSF IPRO, 63I64RSF IPRO) and rust-resistant sources (PI 200492, PI 594538A, PI 587880A, PI 594723, PI 230970, PI 506764, PI 459025A and PI 200487) were evaluated. All F2 plants were individually evaluated in field conditions for ASR phenotypic reactions and classified according to sporulation level. KASP markers were developed according to assays associated with Rpp genes available at SoyBase. Based on a slight difference in map position and different phenotypic disease reactions of PI 200492, we suggest that PI 594723 carries a resistance gene Rpp1-b. The Rpp1-b gene from PI 594723 was mapped on Chr 18 in a 12.4 cM region. The PIs carrying Rpp1-b (PI 594723, PI 587880A, and 594538A) showed strong resistance to ASR compared to the lines carrying Rpp1 (PI 200492). A total of 26 KASP markers were significantly associated (P < 0.01) with ASR resistance. Among those, M1, M5, and M6 (Rpp1), M13 and M14 (Rpp2), M16, M17 and M20 (Rpp3), M25 and M26 (Rpp4), and M27 and M28 (Rpp5) have the potential to be used in marker-assisted selection strategies.

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来源期刊

Bragantia AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

2.40

自引率

8.30%

发文量

审稿时长

4 weeks

期刊介绍： Bragantia é uma revista de ciências agronômicas editada pelo Instituto Agronômico da Agência Paulista de Tecnologia dos Agronegócios, da Secretaria de Agricultura e Abastecimento do Estado de São Paulo, com o objetivo de publicar trabalhos científicos originais que contribuam para o desenvolvimento das ciências agronômicas. A revista é publicada desde 1941, tornando-se semestral em 1984, quadrimestral em 2001 e trimestral em 2005. É filiada à Associação Brasileira de Editores Científicos (ABEC).