Technical note: Testing the effect of different pumping rates on pore-water sampling for ions, stable isotopes, and gas concentrations in the hyporheic zone

Abstract. The hyporheic zone (HZ) is of major importance for carbon and nutrient cycling as well as for the ecological health of stream ecosystems, but it is also a hot spot of greenhouse gas production. Biogeochemical observations in this ecotone are complicated by a very high spatial heterogeneity and temporal dynamics. It is especially difficult to monitor changes in gas concentrations over time because this requires pore-water extraction, which may negatively affect the quality of gas analyses through gas losses or other sampling artifacts. In this field study, we wanted to test the effect of different pumping rates on gas measurements and installed Rhizon samplers for repeated pore-water extraction in the HZ of a small stream. Pore-water sampling at different pumping rates was combined with an optical sensor unit for in situ measurements of dissolved oxygen and a depth-resolved temperature monitoring system. While Rhizon samplers were found to be highly suitable for pore-water sampling of dissolved solutes, measured gas concentrations, here CH4, showed a strong dependency of the pumping rate during sample extraction, and an isotopic shift in gas samples became evident. This was presumably caused by a different behavior of water and gas phase in the pore space. The manufactured oxygen sensor could locate the oxic–anoxic interface with very high precision. This is ecologically important and allows us to distinguish between aerobic and anaerobic processes. Temperature data could not only be used to estimate vertical hyporheic exchange but also depicted sedimentation and erosion processes. Overall, the combined approach was found to be a promising and effective tool to acquire time-resolved data for the quantification of biogeochemical processes in the HZ with high spatial resolution.