高效液相色谱-质谱联用法测定花生基质中白藜芦醇的含量

Q4 Earth and Planetary Sciences

Research Journal of Chemistry and Environment Pub Date : 2023-08-15 DOI:10.25303/2709rjce046052

Vidya Sagar Venkata Pasupuleti, Prashant Bagade, Naga Suresh Reddy Venkata Kachireddy

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引用次数: 0

摘要

建立了一种精确高效的串联质谱(LC-MS/MS)定量花生基质中白藜芦醇的方法。采用hplc -MS/MS分析白藜芦醇，色谱柱为BEH C-18, 130°(50mm 2.1mm, 1.7μm)，流速为0.3 mL/min，流动相为水与乙腈的比例为98:2 (%v/v)与0.1%甲酸的比例为98:2 (v/v)，流动相为乙腈与水的比例为98:2% (v/v)与0.1%甲酸。采用三重四极杆质谱仪和正电喷雾电离系统，在多反应监测(MRM)模式下定量白藜芦醇。对该技术进行了评估，发现能够定量花生样品中的白藜芦醇，从10纳克/克开始，符合SANTE指南。

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A Sensitive and High Throughput LC-MS/MS Method for the Determination of Resveratrol in Peanut Matrix

A precise and very efficient tandem mass spectrometry method (LC-MS/MS) was developed to quantify the resveratrol in the peanut matrix. Two mobile phases made up of water and acetonitrile in a ratio of 98:2 (%v/v) with 0.1% formic acid and acetonitrile and water in a percentage of 98:2 %(v/v) with 0.1% formic acid were used in the LC-MS/MS analysis of resveratrol on a BEH C-18, 130A° (50mm 2.1mm, 1.7μm) analytical column with 0.3 mL/min of flow rate. A triple quadrupole mass spectrometer and a positive electrospray ionization system were used to quantify resveratrol in multiple reaction monitoring (MRM) modes. The technique was evaluated and was found capable of quantifying resveratrol in peanut samples starting at 10 ng/g in compliance with SANTE guidelines.

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来源期刊

Research Journal of Chemistry and Environment 化学-化学综合

CiteScore

0.50

自引率

0.00%

发文量

195

审稿时长

4-8 weeks

期刊介绍： Information not localized