一种高效、低成本的不使用液氮提取大黄属植物DNA的RAPD/ISSR方法

IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology

Research Journal of Biotechnology Pub Date : 2023-08-15 DOI:10.25303/1809rjbt1700174

Anjali Uniyal, Akhilesh Kumar, Vijay Kumar, Sanjay Gupta, Vivek Kumar

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引用次数: 0

摘要

大黄是蓼科重要的药用植物，具有抗菌、通便、抗癌等作用。本研究的目的是规范一种有效和低成本的方法，从北阿坎德邦地区的大黄植物幼叶中提取DNA。该方案包括使用改良的CTAB法提取DNA，而不使用任何液氮。提取缓冲液中NaCl浓度稍高(1.4M)去除多糖，不同比例的β -巯基乙醇用于去除次生代谢物，使其不干扰提取过程。提取的DNA在A260/280 nm处的吸光度比在1.7-2.0的允许范围内，说明DNA纯度高，说明DNA无污染。其浓度约为1485 ng/μl，适于分子基础研究。

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An effective and low-cost method for DNA extraction from Rheum species (A medicinal herb) without using liquid nitrogen for RAPD/ISSR Study

Rheum is an important medicinal plant belonging to family Polygonaceae and is found to have anti-microbial, anti-purgative and anti-cancer properties. The objective of the present study is to standardize an effective and low-cost method for the extraction of DNA from the young juvenile leaves of Rheum plant obtained from the Uttarakhand region. The protocol involved the extraction of DNA using a modified CTAB method without the use of any liquid nitrogen. The concentration of NaCl used in extraction buffer was slightly higher (1.4M) to remove polysaccharides and varying ratios of Beta-mercaptoethanol used to remove the secondary metabolites and keep them away from interfering the extraction process. The absorbance ratio of the extracted DNA at A260/280 nm was within the permissible range of 1.7-2.0, indicating the purity of DNA and showing that the DNA is contamination free. Its concentration was approximately 1485 ng/μl and it was found suitable for molecular-based study.

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来源期刊

Research Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

0.60

自引率

0.00%

发文量

192

审稿时长

1.5 months

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