转42 kDa几丁质酶花生L14在体内条件下生长发育的研究

IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology

Research Journal of Biotechnology Pub Date : 2023-08-15 DOI:10.25303/1809rjbt1750181

Phung Thi Bich Hoa, Nguyen Hoang Tue, Hoang Lan Phuong, Nguyen Xuan Huy, Nguyen Hoang Loc

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引用次数: 0

摘要

本研究的目的是评估转几丁质酶花生对植物致病真菌的生长发育情况，包括含有Chi42基因的WTA-2、含有syncodChi42-1基因的S1A-15和含有syncodChi42-2基因的S2A-12。结果表明，含有曲霉菌SH16 Chi42野生型基因的2个优化基因的花生品系S1A-15 (syncodChi42-1)和S2A-12 (syncodChi42-2)比含有Chi42基因和非转基因对照的wat2品系生长更强，成熟荚果数、百粒重和百粒重更高。此外，品系S1A-15和S2A-12的籽粒脂肪和蛋白质含量也高于WTA-2和对照。这些发现表明，花生根中含有两种合成基因之一的几丁质酶转基因花生可能是抗植物致病真菌花生生产的有希望的候选作物。

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Investigation on growth and development of 42 kDa chitinase transgenic peanuts (Arachis hypogaea L.) cultivar L14 under in vivo condition

The purpose of this study was to assess the growth and development of chitinase transgenic peanuts against phytopathogenic fungi including lines WTA-2 containing the Chi42 gene, S1A-15 containing the syncodChi42-1 gene and S2A-12 containing the syncodChi42-2 gene. The present results show that the peanut lines containing two optimized genes derived from the Chi42 wild-type gene of Trichoderma asperellum SH16, S1A-15 (syncodChi42-1) and S2A-12 (syncodChi42-2) seemed to grow stronger and produce a higher number of mature pods, weight of 100 pods and weight of 100 seeds than line WAT-2 with the Chi42 gene and non-transgenic control. Moreover, lines S1A-15 and S2A-12 also resulted in higher seed quality in terms of lipid and protein content than in comparison to the WTA-2 line and the control. These findings suggest that chitinase transgenic peanuts containing one of two synthetic genes in peanut roots could be a promising candidate for peanut production against phytopathogenic fungi.

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来源期刊

Research Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

0.60

自引率

0.00%

发文量

192

审稿时长

1.5 months

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