水稻内生微生物的特性研究稻瘟病生物防治和植物生长促进剂的潜力

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Shugufta Parveen, Fayaz A. Mohiddin, M. Ashraf Bhat, Zahoor Ahmed Baba, Fehim Jeelani, M. Anwar Bhat, Sajad Un Nabi, Burhan Hamid, Saba Bandey, Farhanaz Rasool, Zakir Amin, Ibrahim Al-Ashkar, Muhammad Adnan, Ayman El Sabagh
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引用次数: 0

摘要

从生长在温带地区的4个著名水稻品种的根、茎和叶中分离出125个内生微生物。研究了其防治稻瘟病和促进植株生长的潜力。双重培养试验表明,5种真菌内生菌(89%-70%)和5种细菌内生菌(72%-61%)具有较强的拮抗活性。随后的检查集中在选定的分离株产生的挥发性化合物上,以对抗爆炸病原体。其中,黄曲霉的几丁质酶(13.76µg mL−1)和铁载体(56.64%)产量最高,嗜麦芽窄养单胞菌的HCN产量最高(36.15µM mL−1)。在促生长性状方面,黄曲霉和阴沟肠杆菌在增磷、产氨、生长素和赤霉素、固氮等方面表现突出。通过分子分析证实了这些内生菌的身份,分别是:非洲哈茨木霉、哈茨木霉、红青霉、黄曲霉、嗜根窄养单胞菌、嗜麦芽窄养单胞菌、蜡样芽孢杆菌、阴沟肠杆菌和地衣芽孢杆菌。在温室条件下,地衣芽孢杆菌(Bacillus licheniformis)和黄芽孢杆菌(A. flavus)的抑菌率最高,为79%,其次是嗜根芽孢杆菌(S. rhizophilia)和非洲芽孢杆菌(T. affroharzianum),分别为77%和73%。综上所述,地衣芽孢杆菌和嗜根窄养单胞菌在水稻稻瘟病防治中具有很大的应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of Endophytic Microorganisms of Rice (Oryza sativa L.) Potentials for Blast Disease Biocontrol and Plant Growth Promoting Agents
One hundred twenty-five endophytic microorganisms were isolated from the roots, stems, and leaves of four prominent rice cultivars growing in temperate regions. Their potential to combat rice blast disease and promote plant growth was investigated. The dual culture tests highlighted the strong antagonistic activity of five fungal (ranging from 89%–70%) and five bacterial (72%–61%) endophytes. Subsequent examination focused on volatile compounds produced by selected isolates to counter the blast pathogen. Among these, the highest chitinase (13.76 µg mL−1) and siderophore (56.64%), was exhibited by Aspergillus flavus, and the highest HCN production was shown by Stenotrophomonas maltophilia (36.15 µM mL−1). In terms of growth promotion traits, Aspergillus flavus and Enterobacter cloacae excelled in activities viz, phosphorous solubilization, ammonia production, auxin and gibberellic acid production, and nitrogen fixation. The Identity of these endophytes was confirmed through molecular analysis as Trichoderma afroharzianum, Trichoderma harzianum, Penicillium rubens, Aspergillus flavus, Stenotrophomonas rhizophila, Stenotrophomonas maltophilia, Bacillus cereus, Enterobacter cloacae, and Bacillus licheniformis. Under greenhouse conditions, the highest disease control was shown by isolate Bacillus licheniformis and A. flavus with an inhibition of 79%, followed by S. rhizophila (77%) and T. afroharzianum (73%). The overall results of this study showed that Bacillus licheniformis and Stenotrophomonas rhizophila have great potential to be used as bio-stimulant and biocontrol agents to manage rice blast disease.
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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