Shugufta Parveen, Fayaz A. Mohiddin, M. Ashraf Bhat, Zahoor Ahmed Baba, Fehim Jeelani, M. Anwar Bhat, Sajad Un Nabi, Burhan Hamid, Saba Bandey, Farhanaz Rasool, Zakir Amin, Ibrahim Al-Ashkar, Muhammad Adnan, Ayman El Sabagh
{"title":"水稻内生微生物的特性研究稻瘟病生物防治和植物生长促进剂的潜力","authors":"Shugufta Parveen, Fayaz A. Mohiddin, M. Ashraf Bhat, Zahoor Ahmed Baba, Fehim Jeelani, M. Anwar Bhat, Sajad Un Nabi, Burhan Hamid, Saba Bandey, Farhanaz Rasool, Zakir Amin, Ibrahim Al-Ashkar, Muhammad Adnan, Ayman El Sabagh","doi":"10.32604/phyton.2023.030921","DOIUrl":null,"url":null,"abstract":"One hundred twenty-five endophytic microorganisms were isolated from the roots, stems, and leaves of four prominent rice cultivars growing in temperate regions. Their potential to combat rice blast disease and promote plant growth was investigated. The dual culture tests highlighted the strong antagonistic activity of five fungal (ranging from 89%–70%) and five bacterial (72%–61%) endophytes. Subsequent examination focused on volatile compounds produced by selected isolates to counter the blast pathogen. Among these, the highest chitinase (13.76 µg mL<sup>−1</sup>) and siderophore (56.64%), was exhibited by <i>Aspergillus flavus</i>, and the highest HCN production was shown by <i>Stenotrophomonas maltophilia</i> (36.15 µM mL<sup>−1</sup>). In terms of growth promotion traits, <i>Aspergillus flavus</i> and <i>Enterobacter cloacae</i> excelled in activities viz, phosphorous solubilization, ammonia production, auxin and gibberellic acid production, and nitrogen fixation. The Identity of these endophytes was confirmed through molecular analysis as Trichoderma afroharzianum, <i>Trichoderma harzianum</i>, <i>Penicillium rubens</i>, <i>Aspergillus flavus</i>, <i>Stenotrophomonas rhizophila</i>, <i>Stenotrophomonas maltophilia</i>, <i>Bacillus cereus</i>, <i>Enterobacter cloacae</i>, and <i>Bacillus licheniformis</i>. Under greenhouse conditions, the highest disease control was shown by isolate <i>Bacillus licheniformis</i> and <i>A. flavus</i> with an inhibition of 79%, followed by <i>S. rhizophila</i> (77%) and <i>T. afroharzianum</i> (73%). The overall results of this study showed that <i>Bacillus licheniformis</i> and <i>Stenotrophomonas rhizophila</i> have great potential to be used as bio-stimulant and biocontrol agents to manage rice blast disease.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization of Endophytic Microorganisms of Rice (Oryza sativa L.) Potentials for Blast Disease Biocontrol and Plant Growth Promoting Agents\",\"authors\":\"Shugufta Parveen, Fayaz A. Mohiddin, M. Ashraf Bhat, Zahoor Ahmed Baba, Fehim Jeelani, M. Anwar Bhat, Sajad Un Nabi, Burhan Hamid, Saba Bandey, Farhanaz Rasool, Zakir Amin, Ibrahim Al-Ashkar, Muhammad Adnan, Ayman El Sabagh\",\"doi\":\"10.32604/phyton.2023.030921\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"One hundred twenty-five endophytic microorganisms were isolated from the roots, stems, and leaves of four prominent rice cultivars growing in temperate regions. Their potential to combat rice blast disease and promote plant growth was investigated. The dual culture tests highlighted the strong antagonistic activity of five fungal (ranging from 89%–70%) and five bacterial (72%–61%) endophytes. Subsequent examination focused on volatile compounds produced by selected isolates to counter the blast pathogen. Among these, the highest chitinase (13.76 µg mL<sup>−1</sup>) and siderophore (56.64%), was exhibited by <i>Aspergillus flavus</i>, and the highest HCN production was shown by <i>Stenotrophomonas maltophilia</i> (36.15 µM mL<sup>−1</sup>). In terms of growth promotion traits, <i>Aspergillus flavus</i> and <i>Enterobacter cloacae</i> excelled in activities viz, phosphorous solubilization, ammonia production, auxin and gibberellic acid production, and nitrogen fixation. The Identity of these endophytes was confirmed through molecular analysis as Trichoderma afroharzianum, <i>Trichoderma harzianum</i>, <i>Penicillium rubens</i>, <i>Aspergillus flavus</i>, <i>Stenotrophomonas rhizophila</i>, <i>Stenotrophomonas maltophilia</i>, <i>Bacillus cereus</i>, <i>Enterobacter cloacae</i>, and <i>Bacillus licheniformis</i>. Under greenhouse conditions, the highest disease control was shown by isolate <i>Bacillus licheniformis</i> and <i>A. flavus</i> with an inhibition of 79%, followed by <i>S. rhizophila</i> (77%) and <i>T. afroharzianum</i> (73%). The overall results of this study showed that <i>Bacillus licheniformis</i> and <i>Stenotrophomonas rhizophila</i> have great potential to be used as bio-stimulant and biocontrol agents to manage rice blast disease.\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32604/phyton.2023.030921\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32604/phyton.2023.030921","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Characterization of Endophytic Microorganisms of Rice (Oryza sativa L.) Potentials for Blast Disease Biocontrol and Plant Growth Promoting Agents
One hundred twenty-five endophytic microorganisms were isolated from the roots, stems, and leaves of four prominent rice cultivars growing in temperate regions. Their potential to combat rice blast disease and promote plant growth was investigated. The dual culture tests highlighted the strong antagonistic activity of five fungal (ranging from 89%–70%) and five bacterial (72%–61%) endophytes. Subsequent examination focused on volatile compounds produced by selected isolates to counter the blast pathogen. Among these, the highest chitinase (13.76 µg mL−1) and siderophore (56.64%), was exhibited by Aspergillus flavus, and the highest HCN production was shown by Stenotrophomonas maltophilia (36.15 µM mL−1). In terms of growth promotion traits, Aspergillus flavus and Enterobacter cloacae excelled in activities viz, phosphorous solubilization, ammonia production, auxin and gibberellic acid production, and nitrogen fixation. The Identity of these endophytes was confirmed through molecular analysis as Trichoderma afroharzianum, Trichoderma harzianum, Penicillium rubens, Aspergillus flavus, Stenotrophomonas rhizophila, Stenotrophomonas maltophilia, Bacillus cereus, Enterobacter cloacae, and Bacillus licheniformis. Under greenhouse conditions, the highest disease control was shown by isolate Bacillus licheniformis and A. flavus with an inhibition of 79%, followed by S. rhizophila (77%) and T. afroharzianum (73%). The overall results of this study showed that Bacillus licheniformis and Stenotrophomonas rhizophila have great potential to be used as bio-stimulant and biocontrol agents to manage rice blast disease.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.