西喜马拉雅不同地区大菱鲆mtCOI遗传多样性评价

Poonam Kumari, Mahender Singh Thakur
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摘要

大黄蜂是全球农业粮食生产和自然生态系统的重要传粉者。它们是探索社会进化、行为和生态的有趣昆虫传粉者,因为它们既有群居的生命周期阶段,也有独居的生命周期阶段,还有一些群居的寄生虫物种。许多关于物种减少的报告列举了病原体传播、栖息地丧失、农药使用和全球温度变化等相互关联的原因。由于对大黄蜂多样性的威胁,我们对少数经过彻底研究的农业授粉物种的依赖是特别危险的。采用Sanger测序技术,对喜玛拉亚西部7个不同地区的野生石榴(Punica granatum)中mtCOI Bombus haemorrhoidalis的遗传多样性进行了分析。Omega结果显示,在痔疮Bombus haemorrhoidalis所有mtCO1序列的比对中,共有6个可变位点。估计COI的过渡/翻转(Ts/Tv)偏差(R)为1.67。与第一和第二密码子位置相比,由于AT富区,核苷酸含量的变化几乎完全存在于第三密码子位置。过渡/翻转(Ts/ Tv)比率在推断自然选择的大小和方向方面具有重要意义。我们的研究清楚地表明,样本物种之间的巨大差异证明了从西喜马拉雅不同地区采集的样本之间存在遗传多样性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluating Genetic Diversity of mtCOI Bombus haemorrhoidalis from Different Regions of Western Himalaya
Bumblebees are a diverse group of crucial pollinators for agricultural food production and natural ecosystems worldwide. They are interesting insect pollinators to explore social evolution, behavior and ecology because they have both eusocial and solitary life-cycle stages as well as some social parasite species. Numerous reports of species declines cite the interrelated causes of pathogen spread, habitat loss, pesticide use and global temperature change. Our reliance on a small number of thoroughly researched species for agricultural pollination is particularly hazardous due to these threats to bumblebee diversity. Sanger sequencing was used to analyze the genetic diversity of mtCOI Bombus haemorrhoidalis, which was collected on Punica granatum (wild pomegranate) from seven different Western Himalayan regions. Omega revealed a total of 6 variable sites in the alignment between all the mtCO1 sequences of Bombus haemorrhoidalis. The estimated transition/transversion (Ts/Tv) bias of COI (R) is 1.67. The variation in nucleotide content almost completely exist in the third codon position due to AT rich region, as compared to the first and second position of codons. The transition/transversion (Ts/ Tv) ratio is significant in deducing the magnitude and direction of natural selection. Our study reveals clearly signifies the vast difference among the sampled species proves that there is genetic diversity between the samples collected from different areas of Western Himalaya.
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