卷曲在睡眠结构中的保守作用

Nicholas R Gessner, Morteza Peiravi, Fan Zhang, Shemsiya Yimam, Danielle Springer, Susan T Harbison
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引用次数: 0

摘要

先前对黑腹果蝇自然变异的研究表明Wnt信号受体在睡眠中卷曲。考虑到Wnt信号通路在物种间高度保守,我们假设frzzered类受体1 (Fzd1), frzzered的小鼠同源物,也可能在睡眠中发挥作用。使用CRISPR转基因方法,我们从C57BL/6N小鼠中去除了大部分Fzd1编码区。我们使用视频实验来测量fzd1缺陷小鼠的睡眠特征。由于已知Wnt信号会影响视觉空间记忆,我们还使用新对象识别(NOR)范式研究了删除对学习和记忆的影响。与对照组相比,fzd1缺陷小鼠的睡眠发生了改变。与对照组相比,小鼠对NOR范式的反应没有不同,但确实表现出类似焦虑的行为。我们的策略表明,对果蝇睡眠自然变异的研究可以转化为小鼠等脊椎动物睡眠的候选基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A conserved role for frizzled in sleep architecture
Abstract Previous studies of natural variants in Drosophila melanogaster implicated the Wnt signaling receptor frizzled in sleep. Given that the Wnt signaling pathway is highly conserved across species, we hypothesized that frizzled class receptor 1 (Fzd1), the murine homolog of frizzled, would also have a role in sleep. Using a CRISPR transgenic approach, we removed most of the Fzd1 coding region from C57BL/6N mice. We used a video assay to measure sleep characteristics in Fzd1-deficient mice. As Wnt signaling is known to affect visuospatial memory, we also examined the impact of the deletion on learning and memory using the Novel Object Recognition (NOR) paradigm. Fzd1-deficient mice had altered sleep compared to littermate controls. The mice did not respond differently to the NOR paradigm compared to controls but did display anxiety-like behavior. Our strategy demonstrates that the study of natural variation in Drosophila sleep translates into candidate genes for sleep in vertebrate species such as the mouse.
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