紫杉对uva照射下正常人表皮角质形成细胞的抗衰老作用

Q4 Pharmacology, Toxicology and Pharmaceutics

Korean Journal of Pharmacognosy Pub Date : 2023-09-30 DOI:10.22889/kjp.2023.54.3.96

Joong Hyun Shim

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引用次数: 0

摘要

本研究旨在探讨山茱萸提取物对正常人表皮角质形成细胞(NHEKs)的抗衰老和保湿作用。采用实时定量RT-PCR检测细胞活力、与皮肤水化相关的mRNA水平、透明质酸(HA)-ELISA检测HA的产生，检测枸杞提取物对NHEKs的保湿作用。C. costata提取物增加了uva辐照NHEKs中AQP3和HAS2基因的mRNA水平和HA的产生。另一方面，荆芥提取物降低了NHEKs中分化角质细胞标志物FLG、KRT1和KRT10的基因表达。本研究证实了肋草提取物的保湿作用。结果表明，花楸提取物可作为皮肤保湿和抗衰老产品的功能成分。在药妆品、食品和药品等方面，还需要进一步的研究。

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Anti-aging Effect of Carpomitra costata in UVA-irradiated Normal Human Epidermal Keratinocytes

This research was conducted to investigate the anti-aging and moisturizing effects Carpomitra costata extract on normal human epidermal keratinocytes (NHEKs). Moisturizing effects of C. costata extract on NHEKs were determined by quantitative real-time RT-PCR to identify cell viability assays, the mRNA levels associated with skin hydration, and hyaluronic acid (HA)-ELISA to detect HA production. C. costata extract increased mRNA levels of AQP3 and HAS2 genes and HA production in UVA-irradiated NHEKs. On the other hand, C. costata extract decreased the gene expressions of the FLG, KRT1 and KRT10 known as differentiated keratinocyte marker in NHEKs. This research showed the moisturizing effects of C. costata extract. The results indicate that C. costata extract can be a functional ingredient in skin moisturizing and anti-aging products. Further study is warranted regarding the use of C. costata extract to develop not only cosmeceutics but also food and medicine.

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来源期刊

Korean Journal of Pharmacognosy Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.70

自引率

0.00%

发文量