人巨细胞病毒UL138显性b细胞表位的筛选鉴定及其临床应用

IF 2.1 4区医学 Q3 VIROLOGY

Future Virology Pub Date : 2023-08-01 DOI:10.2217/fvl-2023-0052

Hua Zhu, Xuanlu Xu, Jing Jiang, Xiangyang Xue, Wenmiao Zhang, Ruanmin Zou

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引用次数: 0

摘要

目的:预测人巨细胞病毒UL138基因的显性b细胞表位，为其潜伏感染提供新的检测方法。方法:利用预测的优势表位构建重组质粒，诱导其在小鼠体内表达和免疫，鉴定多肽抗原性、免疫原性和人血清。结果:获得5个潜在显性b细胞表位。新生儿、成人和小鼠血清标本的检出率明显高于人巨细胞病毒(HCMV) (IgG、IgM)和UL138基因DNA和cDNA检测(p <0.05)。结论:预测的显性b细胞表位对检测人血清HCMV潜伏感染具有较高的敏感性，可替代传统的HCMV IgG检测方法。

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Screening and identification of dominant B-cell epitopes of human cytomegalovirus UL138 and its clinical applications

Aim: To predict the dominant B-cell epitope of the human cytomegalovirus UL138 gene and to provide a new assay for its latent infection. Methods: Recombinant plasmids were constructed with the predicted dominant epitopes, induced to be expressed and immunized in mice as a way to identify peptide antigenicity, immunogenicity and human serum. Results: Five potentially dominant B-cell epitopes were obtained. The positive rate of detection was found to be significantly higher in neonatal, adult and mouse serum specimens than in human cytomegalovirus (HCMV) (IgG, IgM) and UL138 gene DNA and cDNA assays (p < 0.05). Conclusion: The predicted dominant B-cell epitope is highly sensitive for the detection of human serum HCMV latent infection and can replace the traditional HCMV IgG assay.

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来源期刊

Future Virology 医学-病毒学

CiteScore

4.00

自引率

3.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Future Virology is a peer-reviewed journal that delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research. It is an interdisciplinary forum for all scientists working in the field today.