富萜类化合物齿菜的化学预防措施及氧化应激效应。紫外光b诱导的体外皮肤癌变模型中的叶提取物(TRCO)

Q3 Pharmacology, Toxicology and Pharmaceutics
Muhammad Wahizul Haswan Abdul Aziz, Dayang Fredalina Basri, Siti Fathiah Masre, Ahmad Rohi Ghazali
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引用次数: 0

摘要

在人类外层和中层皮肤中发现的最常见的癌症类型是角化细胞癌,通常被称为非黑色素瘤皮肤癌。虽然皮肤癌具有侵袭性,但通常不会致命。本研究旨在检测富含萜类化合物的齿菜鸭的化学预防和氧化应激作用。黄芪叶提取物(TRCO)在uvb诱导的皮肤癌模型中的作用。采用500µg/ml TRCO (TRCO500)预处理,对涉及人角质形成细胞(HaCaT)的模型进行优化;1000µg/ml TRCO (TRCO1000)。根据我们的研究结果,HaCaT致癌转化模型的重复UVB暴露导致细胞形状改变。预处理TRCO1000可显著降低uvb诱导的HaCaT中肿瘤蛋白p53 (TP53)和增殖标志物KI-67 (KI67)的表达。TRCO治疗导致氧化应激的总体减少。TRCO预处理降低了脂质过氧化物和LPO,并显著降低了蛋白质羰基。综上所述,利用TRCO对体外皮肤癌变模型进行化学预防研究的良好结果表明,值得对牙齿草中萜类化合物进行进一步的分离和评估。作为一种可能的化学预防剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chemopreventive Measurements and Oxidative Stress Effects of Terpenoid-rich Canarium odontophyllum Miq. Leaf Extract (TRCO) in Ultraviolet B-Induced In-Vitro Skin Carcinogenesis Model
The most common type of cancer found in the human outer and middle skin layers is keratinocyte carcinoma, often known as non-melanoma skin cancer. Although it can be aggressive, skin cancer is generally not fatal. This study aims to measure the chemopreventive and oxidative stress effects of terpenoid-rich Canarium odontophyllum Miq.’s leaf extract (TRCO) in the UVB-induced skin carcinogenesis model. The model involving human keratinocytes (HaCaT) was adapted with optimisations with pretreatment of 500 µg/ml TRCO (TRCO500) & 1000 µg/ml TRCO (TRCO1000). The HaCaT oncogenic transformation model's repetitive UVB exposure resulted in altered cell shape, according to our findings. Pretreatment of TRCO1000 significantly reduced tumour protein p53 (TP53) and marker of proliferation KI-67 (KI67) expression in UVB-induced HaCaT. Treatment with TRCO resulted in an overall reduction in oxidative stress. TRCO pretreatment showed reduced lipid peroxides, LPO and significantly reduced protein carbonyls. In conclusion, the promising results of this chemopreventive study using TRCO on this in-vitro skin carcinogenesis model suggested that it is worthwhile to conduct further isolations and assessments of terpenoids from C. odontophyllum Miq. leaf as a possible chemoprevention agent.
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来源期刊
Biomedical and Pharmacology Journal
Biomedical and Pharmacology Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology
CiteScore
1.20
自引率
0.00%
发文量
189
期刊介绍: Biomedical and Pharmacology Journal (BPJ) is an International Peer Reviewed Research Journal in English language whose frequency is quarterly. The journal seeks to promote research, exchange of scientific information, consideration of regulatory mechanisms that affect drug development and utilization, and medical education. BPJ take much care in making your article published without much delay with your kind cooperation and support. Research papers, review articles, short communications, news are welcomed provided they demonstrate new findings of relevance to the field as a whole. All articles will be peer-reviewed and will find a place in Biomedical and Pharmacology Journal based on the merit and innovativeness of the research work. BPJ hopes that Researchers, Research scholars, Academician, Industrialists etc. would make use of this journal for the development of science and technology. Topics of interest include, but are not limited to: Biochemistry Genetics Microbiology and virology Molecular, cellular and cancer biology Neurosciences Pharmacology Drug Discovery Cardiovascular Pharmacology Neuropharmacology Molecular & Cellular Mechanisms Immunology & Inflammation Pharmacy.
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