Muhammad Alamsyah Aziz, Sofie Rifayani Krisnadi, Budi Handono, Budi Setiabudiawan
{"title":"孕妇子宫肌瘤1-41细胞活力试验对维生素D的应用","authors":"Muhammad Alamsyah Aziz, Sofie Rifayani Krisnadi, Budi Handono, Budi Setiabudiawan","doi":"10.15850/amj.v10n3.2750","DOIUrl":null,"url":null,"abstract":"Background: Preterm labor is one of the universal causes of perinatal mortality worldwide. One of the causes of preterm labor is uterine muscle integrity problems. Some mechanistic studies show insight into vitamin D activity’s possible role in the injured muscle. This study aimed to determine whether vitamin D can increase muscle cell viability.Methods: This experimental research used human smooth muscle uterine myometrium cell line PHM1-41. The cells were cultured for 24 hours in hypoxia condition, then incubated with several doses of vitamin D. The PHM1-41 cell viability was measured using spectrophotometry.Results: The result showed that the minimum level of muscle cell viability after vitamin D incubation was with 300nM administration, and the maximum level was after 10nM (88.57%+4.48 and 96.21%+2.13 respectively).Conclusions: Vitamin D at a specific dose could improve cell availability. The optimal dose to improve cell viability was 10nM.","PeriodicalId":31310,"journal":{"name":"Althea Medical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pregnant Human Myometrial 1-41 Cell Viability Test on Vitamin D Administration\",\"authors\":\"Muhammad Alamsyah Aziz, Sofie Rifayani Krisnadi, Budi Handono, Budi Setiabudiawan\",\"doi\":\"10.15850/amj.v10n3.2750\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Preterm labor is one of the universal causes of perinatal mortality worldwide. One of the causes of preterm labor is uterine muscle integrity problems. Some mechanistic studies show insight into vitamin D activity’s possible role in the injured muscle. This study aimed to determine whether vitamin D can increase muscle cell viability.Methods: This experimental research used human smooth muscle uterine myometrium cell line PHM1-41. The cells were cultured for 24 hours in hypoxia condition, then incubated with several doses of vitamin D. The PHM1-41 cell viability was measured using spectrophotometry.Results: The result showed that the minimum level of muscle cell viability after vitamin D incubation was with 300nM administration, and the maximum level was after 10nM (88.57%+4.48 and 96.21%+2.13 respectively).Conclusions: Vitamin D at a specific dose could improve cell availability. The optimal dose to improve cell viability was 10nM.\",\"PeriodicalId\":31310,\"journal\":{\"name\":\"Althea Medical Journal\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Althea Medical Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15850/amj.v10n3.2750\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Althea Medical Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15850/amj.v10n3.2750","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Pregnant Human Myometrial 1-41 Cell Viability Test on Vitamin D Administration
Background: Preterm labor is one of the universal causes of perinatal mortality worldwide. One of the causes of preterm labor is uterine muscle integrity problems. Some mechanistic studies show insight into vitamin D activity’s possible role in the injured muscle. This study aimed to determine whether vitamin D can increase muscle cell viability.Methods: This experimental research used human smooth muscle uterine myometrium cell line PHM1-41. The cells were cultured for 24 hours in hypoxia condition, then incubated with several doses of vitamin D. The PHM1-41 cell viability was measured using spectrophotometry.Results: The result showed that the minimum level of muscle cell viability after vitamin D incubation was with 300nM administration, and the maximum level was after 10nM (88.57%+4.48 and 96.21%+2.13 respectively).Conclusions: Vitamin D at a specific dose could improve cell availability. The optimal dose to improve cell viability was 10nM.