余甘子茎皮提取物体外抗氧化活性、生药评价、HPTLC和FTIR指纹图谱研究

Q3 Pharmacology, Toxicology and Pharmaceutics

Biomedical and Pharmacology Journal Pub Date : 2023-09-30 DOI:10.13005/bpj/2716

Zeba Siddiqui, Mohammad Irfan Khan, Badruddeen Badruddeen, Juber Akhtar, Mohammad Ahmed

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引用次数: 0

摘要

背景:为了安全有效地使用天然药物，对原料药的质量控制至关重要。由于缺乏科学的质量控制、植物化学和药理性质的研究，因此，Skeels，本研究是进行的。目的:建立余甘子茎皮的各种生药和植物化学参数，包括宏观和微观研究，以及HPTLC和FTIR指纹图谱分析，以及总酚和总黄酮含量，并探讨其抗氧化潜力。方法:采用各种比色法测定总酚和总黄酮含量。DPPH法测定其抗氧化活性。进行了理化分析，并使用各种技术(如HPTLC、FTIR分析和比色法)确定了各种官能团的存在。结果:植物化学筛选结果显示，黄酮类、单宁类、萜类、酚类、碳水化合物等多种植物成分均存在。在各种溶剂体系中。总酚含量为189.74±0.52mg GAE/g，类黄酮含量为38.92±0.47 mg QE/g。重金属浓度在可接受范围内。与抗坏血酸的IC50值31.82相比，茎皮在甲醇和水提取物中的IC50值分别为26.92和26.52，具有相当的抗氧化活性。HPTLC指纹图谱设想了余甘子中几种植物成分的存在。FTIR分析证实了酚类官能团的存在。结论:本研究为原料中掺假的检测和管理提供了参考。该研究还提供了深入了解这种植物的抗氧化特性，验证了其作为天然抗氧化剂的民族药理学用途。

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In Vitro Antioxidant Activity, Pharmacognostical Evaluation, HPTLC and FTIR Fingerprinting of Phyllanthus Acidus L. Stem Bark Extract for Better Application in Phytotherapy

Background: For safe and efficacious use of natural products, quality control of the crude drug is of paramount importance. Due to lack of scientific investigations pertaining to quality control phytochemical and pharmacological properties of Phyllanthus acidus (L.) Skeels, the present study was undertaken. Objective: The aim of the study is to establish various pharmacognostical and phytochemical parameters, including both macro and microscopic studies, along with HPTLC and FTIR fingerprinting analysis, total phenolic and flavonoid content of stem bark of Phyllanthus acidus, followed by exploring its anti-oxidant potential. Method: The determination of total phenolic and flavonoid content was done by various colorimetric assays. DPPH assay was used to establish antioxidant activity. Physiochemical analysis was carried out and presence of various functional groups was determined using various techniques like HPTLC, FTIR analysis and colorimetric assays. Result: The phytochemical screening showed the presence of various phytoconstituents like phenols, carbohydrates, flavonoid, tannins and terpenoids. in various solvent systems. Total phenolic was established as 189.74±0.52mg GAE/g and flavonoid content was found to be38.92±0.47 mg QE/g. Concentration of heavy metal was within acceptable limits. The stem bark showed comparable antioxidant activity in methanolic and aqueous extract showed IC50 values of 26.92 and 26.52 respectively compared to ascorbic acid having IC50 value of 31.82. HPTLC fingerprinting envisaged the presence of several phytoconstituents in Phyllanthus acidus. The FTIR analysis established the presence of phenolic functional groups. Conclusion: The referential information provided by this study will be useful to determine and manage adulterations in raw material. The study also provides insight into antioxidant property of this plant validating its ethno pharmacological use as a natural antioxidant.

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来源期刊

Biomedical and Pharmacology Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

1.20

自引率

0.00%

发文量

189

期刊介绍： Biomedical and Pharmacology Journal (BPJ) is an International Peer Reviewed Research Journal in English language whose frequency is quarterly. The journal seeks to promote research, exchange of scientific information, consideration of regulatory mechanisms that affect drug development and utilization, and medical education. BPJ take much care in making your article published without much delay with your kind cooperation and support. Research papers, review articles, short communications, news are welcomed provided they demonstrate new findings of relevance to the field as a whole. All articles will be peer-reviewed and will find a place in Biomedical and Pharmacology Journal based on the merit and innovativeness of the research work. BPJ hopes that Researchers, Research scholars, Academician, Industrialists etc. would make use of this journal for the development of science and technology. Topics of interest include, but are not limited to: Biochemistry Genetics Microbiology and virology Molecular, cellular and cancer biology Neurosciences Pharmacology Drug Discovery Cardiovascular Pharmacology Neuropharmacology Molecular & Cellular Mechanisms Immunology & Inflammation Pharmacy.