Natalia Kholod, Andrey Koltsov, Nikola Vaskovic, Galina Koltsova
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Identification of endoplasmic reticulum stress response genes in homologous vs. heterologous asf infections in vitro
Abstract The endoplasmic reticulum (ER) is crucial for the production, processing and transport of proteins. Infection with pathogens activates Unfolded Protein Response (UPR), which can lead to their survival/replication or elimination from the body. Although little is known about the role of the ER stress response in the pathogenesis of viral infections, the regulation of ER stress may be important in intractable infectious diseases. We conducted a comparative analysis of the expression of genes involved in ER stress response in peripheral blood mononuclear cells (PBMCs) from animals immunized with an attenuated strain of ASFV strain Congo-a (KK262) and then stimulated in vitro by two serologically different virulent strains Congo-v (K49) or Mozambique-v (M78), to expand our understanding of the early determinants of response to homologous and heterologous infection. We found up-regulation of genes of all three sensory molecules (PERK, ATF6 and IRE1) of UPR pathway in cells infected with only a homologous strain. For the first time, a number of up-regulated genes of the ER-associated degradation pathway (ERAD), which destroys misfolded proteins, were also detected. By understanding how viruses modify elements of cellular response to stress, we learn more about the pathogenesis, as well as how we can use it to prevent viral diseases.
期刊介绍:
The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.