同源与异源非洲猪瘟体外感染内质网应激反应基因的鉴定

IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES
Natalia Kholod, Andrey Koltsov, Nikola Vaskovic, Galina Koltsova
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引用次数: 0

摘要

内质网(ER)在蛋白质的产生、加工和运输中起着至关重要的作用。病原体感染激活未折叠蛋白反应(UPR),这可以导致它们的生存/复制或从体内消除。虽然内质网应激反应在病毒感染发病机制中的作用知之甚少,但内质网应激的调节可能在难治性传染病中很重要。我们比较分析了用ASFV刚果-a减毒株(KK262)免疫的动物外周血单核细胞(PBMCs)中参与内质网应激反应的基因的表达,然后在体外用两种血清学上不同的毒力株刚果-v (K49)或莫桑比克-v (M78)刺激,以扩大我们对同源和异源感染反应的早期决定因素的理解。我们发现,在仅感染一株同源菌株的细胞中,UPR通路的三个感觉分子(PERK、ATF6和IRE1)的基因均上调。研究人员还首次发现了er相关降解途径(ERAD)的一些上调基因,ERAD可以破坏错误折叠的蛋白质。通过了解病毒如何改变细胞对压力的反应,我们了解了更多的发病机制,以及我们如何利用它来预防病毒性疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of endoplasmic reticulum stress response genes in homologous vs. heterologous asf infections in vitro
Abstract The endoplasmic reticulum (ER) is crucial for the production, processing and transport of proteins. Infection with pathogens activates Unfolded Protein Response (UPR), which can lead to their survival/replication or elimination from the body. Although little is known about the role of the ER stress response in the pathogenesis of viral infections, the regulation of ER stress may be important in intractable infectious diseases. We conducted a comparative analysis of the expression of genes involved in ER stress response in peripheral blood mononuclear cells (PBMCs) from animals immunized with an attenuated strain of ASFV strain Congo-a (KK262) and then stimulated in vitro by two serologically different virulent strains Congo-v (K49) or Mozambique-v (M78), to expand our understanding of the early determinants of response to homologous and heterologous infection. We found up-regulation of genes of all three sensory molecules (PERK, ATF6 and IRE1) of UPR pathway in cells infected with only a homologous strain. For the first time, a number of up-regulated genes of the ER-associated degradation pathway (ERAD), which destroys misfolded proteins, were also detected. By understanding how viruses modify elements of cellular response to stress, we learn more about the pathogenesis, as well as how we can use it to prevent viral diseases.
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来源期刊
Acta Veterinaria-Beograd
Acta Veterinaria-Beograd 农林科学-兽医学
CiteScore
1.30
自引率
16.70%
发文量
33
审稿时长
18-36 weeks
期刊介绍: The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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