利用SSR标记对面包小麦(Triticum aestivum)基因型进行分子分析

IF 0.3 4区农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY

Indian Journal of Agricultural Sciences Pub Date : 2023-09-26 DOI:10.56093/ijas.v93i9.139173

SUMAN DEVI, VIKRAM SINGH, SHIKHA YASHVEER, MOHINDER SINGH DALAL, None PARAS, RUKOO CHAWLA, DARYA KHAN AKBARZAI, HARSH CHAURASIA

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引用次数: 0

摘要

利用43个多态性SSR标记，于2019-20和2020-21冬季在哈里亚纳邦农业大学研究农场对80个面包小麦(Triticum aestivum L.)基因型的遗传多样性进行了研究。共发现84个等位基因，平均每个位点扩增3个等位基因。等位基因PIC的平均值为0.26 ~ 0.82。引物，即Xgwm 129、Xgwm 131、TaGST、CFA2147、Xwmc48、Xbarc 1165和Xwmc169，由于它们的高PIC值，可以被认为是特别有用的。不同指数变化范围为0.14 ~ 0.42。利用多态标记分子数据构建的树状图，将80个小麦基因型分为35个和45个两大类。利用STRUCTURE将基因型划分为4个主要亚群，Fst值分别为0.351、0.363、0.508和0.313。未来小麦品种抗非生物胁迫育种应考虑基因型聚类。分子遗传多样性评估是一种可靠的鉴定品种的方法，通过分析品种DNA的特定区域来确定其独特的遗传谱。

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Molecular characterization of bread wheat (Triticum aestivum) genotypes using SSR markers

An experiment was conducted during winter (rabi) seasons of 2019–20 and 2020–21 at the research farm of CCS Haryana Agricultural University to study the genetic diversity of 80 bread wheat (Triticum aestivum L.) genotypes, using 43 polymorphic SSR markers. A total of 84 alleles were discovered, with an average of 3 alleles amplified per locus. The average value of the allelic PIC varied from 0.26 to 0.82. Primers, viz. Xgwm 129, Xgwm 131, TaGST, CFA2147, Xwmc48, Xbarc 1165 and Xwmc169 may be deemed particularly informative given their high PIC values. Indices of dissimilarity varied from 0.14 to 0.42. Eighty wheat genotypes were clustered into two main groups with 35 and 45 genotypes each using the dendrogram constructed on the basis of molecular data of polymorphic markers. Using STRUCTURE, genotypes were classified into 4 major sub-populations having Fst values 0.351, 0.363, 0.508 and 0.313, respectively. Future breeding operations in wheat cultivars for tolerance to abiotic stress should consider genotypes clustering into different groups. Assessing the molecular genetic diversity is a reliable approach to identify cultivars by analyzing of specific regions of the cultivars DNA based on their unique genetic profiles.

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来源期刊

Indian Journal of Agricultural Sciences 农林科学-农业综合

CiteScore

0.80

自引率

25.00%

发文量

273

审稿时长

6 months

期刊介绍： The Indian Journal of Agricultural Sciences publishes papers concerned with the advancement of agriculture throughout the world. It publishes original scientific work related to strategic and applied studies in all aspects of agricultural science and exploited species, as well as reviews of scientific topics of current agricultural relevance. Specific topics of interest include (but are not confined to): genetic resources, all aspects of crop improvement,crop production,crop protection, physiology, modeling of crop systems, the scientific underpinning of agronomy, engineering solutions, decision support systems, land use, environmental impacts of agriculture and forestry, impacts of climate change, rural biodiversity, experimental design and statistical analysis, the application of new analytical and study methods (including molecular studies) and agricultural economics. The journal also publishes book reviews. Articles are accepted on the following broad disciplines: Agric. Engineering & Technology, Agric. Social & Economic Sci., Agronomy, Biometry, Biosciences, Cytology, Ecology, Environmental Sciences, Fertilization, Forestry , Genetics, Horticultural Sciences, Microbiology, Pest, Weed Control etc., Molecular Biology, Plant Pathology, Plant Breeding, Physiology and Biochemistry, Soil Sciences, Special Cultivation Technology, Stress Breeding, Agric. extension, and Cell Biology.