{"title":"多产入侵植物水螅的环境DNA检测范围","authors":"Daniel Weber, Michael Tessler, Steven Pearson","doi":"10.1086/728097","DOIUrl":null,"url":null,"abstract":"Environmental DNA (eDNA) is a powerful conservation tool that has made significant advancements in the past decade. Since its initial application in 2008 to detect invasive tadpoles, eDNA has become popular for finding rare and invasive species that are otherwise difficult to detect through traditional field sampling methods. This study sought to determine a detectable range of eDNA for an aggressively invasive aquatic plant species, Hydrilla verticillata (L. f.) Royle, as well as to understand how stream discharge affects eDNA detection distance. To do so, we surveyed downstream of a large reservoir, which is separated from its distributary by a dam. Hydrilla verticillata is present in the reservoir but absent in the distributary. We used correlation analysis to understand the relationship between transportation distance and detection. We also built upon a model developed by Pont et al. (2018) to assess the relationships between stream discharge and eDNA detection distance, as well as to compare this relationship for our plant-based eDNA study with previous animal-based studies. By utilizing this natural system and surveying at different points downstream of the known population, this study detected H. verticillata eDNA nearly 5 km from the source population. This study, which is the 1st known instance of correlating plant eDNA to animal eDNA detection ranges, suggests the genetic material of both kingdoms are similarly detectable in flowing freshwaters and supports other studies that suggest discharge is a strong predictor of the detectable distance of eDNA.","PeriodicalId":48926,"journal":{"name":"Freshwater Science","volume":"8 1","pages":"0"},"PeriodicalIF":1.7000,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Environmental DNA detection range for hydrilla, a prolific invasive plant\",\"authors\":\"Daniel Weber, Michael Tessler, Steven Pearson\",\"doi\":\"10.1086/728097\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Environmental DNA (eDNA) is a powerful conservation tool that has made significant advancements in the past decade. Since its initial application in 2008 to detect invasive tadpoles, eDNA has become popular for finding rare and invasive species that are otherwise difficult to detect through traditional field sampling methods. This study sought to determine a detectable range of eDNA for an aggressively invasive aquatic plant species, Hydrilla verticillata (L. f.) Royle, as well as to understand how stream discharge affects eDNA detection distance. To do so, we surveyed downstream of a large reservoir, which is separated from its distributary by a dam. Hydrilla verticillata is present in the reservoir but absent in the distributary. We used correlation analysis to understand the relationship between transportation distance and detection. We also built upon a model developed by Pont et al. (2018) to assess the relationships between stream discharge and eDNA detection distance, as well as to compare this relationship for our plant-based eDNA study with previous animal-based studies. By utilizing this natural system and surveying at different points downstream of the known population, this study detected H. verticillata eDNA nearly 5 km from the source population. This study, which is the 1st known instance of correlating plant eDNA to animal eDNA detection ranges, suggests the genetic material of both kingdoms are similarly detectable in flowing freshwaters and supports other studies that suggest discharge is a strong predictor of the detectable distance of eDNA.\",\"PeriodicalId\":48926,\"journal\":{\"name\":\"Freshwater Science\",\"volume\":\"8 1\",\"pages\":\"0\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2023-10-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Freshwater Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1086/728097\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Freshwater Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1086/728097","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ECOLOGY","Score":null,"Total":0}
Environmental DNA detection range for hydrilla, a prolific invasive plant
Environmental DNA (eDNA) is a powerful conservation tool that has made significant advancements in the past decade. Since its initial application in 2008 to detect invasive tadpoles, eDNA has become popular for finding rare and invasive species that are otherwise difficult to detect through traditional field sampling methods. This study sought to determine a detectable range of eDNA for an aggressively invasive aquatic plant species, Hydrilla verticillata (L. f.) Royle, as well as to understand how stream discharge affects eDNA detection distance. To do so, we surveyed downstream of a large reservoir, which is separated from its distributary by a dam. Hydrilla verticillata is present in the reservoir but absent in the distributary. We used correlation analysis to understand the relationship between transportation distance and detection. We also built upon a model developed by Pont et al. (2018) to assess the relationships between stream discharge and eDNA detection distance, as well as to compare this relationship for our plant-based eDNA study with previous animal-based studies. By utilizing this natural system and surveying at different points downstream of the known population, this study detected H. verticillata eDNA nearly 5 km from the source population. This study, which is the 1st known instance of correlating plant eDNA to animal eDNA detection ranges, suggests the genetic material of both kingdoms are similarly detectable in flowing freshwaters and supports other studies that suggest discharge is a strong predictor of the detectable distance of eDNA.
期刊介绍:
Freshwater Science (FWS) publishes articles that advance understanding and environmental stewardship of all types of inland aquatic ecosystems (lakes, rivers, streams, reservoirs, subterranean, and estuaries) and ecosystems at the interface between aquatic and terrestrial habitats (wetlands, riparian areas, and floodplains). The journal regularly features papers on a wide range of topics, including physical, chemical, and biological properties of lentic and lotic habitats; ecosystem processes; structure and dynamics of populations, communities, and ecosystems; ecology, systematics, and genetics of freshwater organisms, from bacteria to vertebrates; linkages between freshwater and other ecosystems and between freshwater ecology and other aquatic sciences; bioassessment, conservation, and restoration; environmental management; and new or novel methods for basic or applied research.