ELISA与real-time PCR检测乳腺癌牙周炎患者HSV-1抗原的比较

Saif Salahuddin Jasim, None Ghada Ibrahim Taha
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摘要

J Fac Med巴格达2023;第65卷第3期收稿日期:3月背景:牙周炎是一种长期的感染,它会破坏牙龈、牙周韧带和支撑牙齿的牙槽骨。牙龈炎症和慢性牙周炎都是由牙菌斑中的细菌和疱疹病毒引起的,尤其是单纯疱疹病毒的1型和2型。目的:比较ELISA和real-time PCR在乳腺癌牙周炎化疗患者单纯疱疹病毒检测中的应用。病例与方法:本病例-对照研究共90例,其中30例为化疗后牙周炎合并乳腺癌患者,30例为牙周炎(未化疗)患者,30例为无牙周炎(健康牙龈)、无乳腺癌患者作为健康对照组。平均±SD年龄为(51.06±8.521)岁。采用酶联免疫吸附试验(ELISA)试剂盒和实时荧光定量PCR检测唾液样品中的单纯疱疹病毒1型。结果:在本研究中,接受化疗的牙周炎患者中HSV-1 Ag的发病率较高,但与未接受化疗的牙周炎患者和健康对照组相比差异不显著。而Real-time PCR结果分别为阳性(13.3%)和阳性(10.0%),说明大多数未化疗的牙周炎患者和接受化疗的牙周炎患者存在HSV,但差异不显著(p>0.05)。结论:Real-time PCR能够支持ELISA的研究结果。由于ELISA比实时PCR更具成本效益,建议先用ELISA进行筛选,然后用实时PCR对阳性样本进行调查,以确认是否存在HSV-DNA。, 2023录用日期:2023年6月出版日期:2023年10月
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison between HSV-1 Ag detection techniques by ELISA and real-time PCR in breast cancer patients suffering from periodontitis
J Fac Med Baghdad 2023; Vol.65, No. 3 Received:March., 2023 Accepted: June. 2023 Published: Oct. 2023 Background: Periodontitis is a long-standing infection that destroys the gums, periodontal ligaments, and the alveolar bone that supports the teeth. Inflammation of the gums and chronic periodontitis are both caused by the bacteria in the dental plaque and the herpes viruses, especially types 1 and 2 of the herpes simplex virus. Objectives: To compare the ELISA and real-time PCR as ways to detect the herpes simplex virus in breast cancer patients with periodontitis who are receiving chemotherapy. Cases and methods: In this case-control study, a total of 90 subjects (30 with periodontitis with breast cancer after receiving chemotherapy, 30 with periodontitis (without chemotherapy treatment), and 30 cases which do not have periodontitis (healthy gingiva) and without breast cancer as a healthy control group. The mean ±SD age was (51.06±8.521). Enzyme-linked immunosorbent assay (ELISA) kits were used for the analysis and Real-Time PCR to detection of Herpes simplex virus-1 in saliva samples. Results: In the present study, the incidence of HSV-1 Ag in patients having periodontitis and receiving chemotherapy was high but not significantly so compared with those having periodontitis but without chemotherapy and the healthy control group. In contrast, the result of Real-time PCR was positive (13.3%) and (10.0%), which proves the presence of HSV in most patients with periodontitis without chemotherapy and with periodontitis receiving chemotherapy but was not significant (p>0.05). Conclusion: Real-time PCR was able to back up ELISA's findings in this study. Since ELISA is more cost-effective than real-time PCR, it is advised that the first screening may be performed using ELISA, and then positive samples might be investigated with real-time PCR to confirm the presence of HSV-DNA Received:March., 2023 Accepted: June. 2023 Published: Oct. 2023
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