金、银纳米颗粒对尿路致病性大肠杆菌的抗菌和抗生物膜作用通过扫描电子显微镜(SEM)分析

IF 0.2 Q4 HEALTH CARE SCIENCES & SERVICES
Rini Purbowati, Vania Mitha Pratiwi, Masfufatun Masfufatun, Putu Oky Ari Tania, Ali Khumaeni
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引用次数: 0

摘要

尿路致病性大肠杆菌(UPEC)是一种与尿路感染和生物膜形成有关的医院病原菌,它有助于抗生素耐药性。发现有效的抗菌剂至关重要。本研究旨在利用扫描电子显微镜(SEM)研究金、银纳米颗粒对UPEC的抗菌和抗生物膜作用。UPEC生物膜在硝化纤维素膜上37℃培养48小时,然后用金纳米粒子(50 ppm和100 ppm)和银纳米粒子(50 ppm和100 ppm)处理48小时。通过细胞密度和扫描电镜分析来评价抗菌和抗生物膜活性。扫描电镜显示,纳米颗粒处理后,细胞密度降低,生物膜形成减少,细胞形态改变,表面粗糙,皱褶。综上所述,金纳米粒子和银纳米粒子表现出抗菌和抗生物膜的特性。扫描电镜是研究纳米金和银对细菌细胞形态和生物膜种群的抗菌作用的重要工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibacterial and antibiofilm effects of gold and silver nanoparticles against the uropathogenic <i>Escherichia coli</i> by scanning electron microscopy (SEM) analysis
Uropathogenic Escherichia coli (UPEC) is a nosocomial pathogen associated with urinary tract infections and biofilm formation, which contributes to antibiotic resistance. Discovering potent antibacterial agents is crucial. This study aimed to assess the antibacterial and antibiofilm effects of gold and silver nanoparticles on UPEC using Scanning Electron Microscopy (SEM). UPEC biofilms were cultivated on nitrocellulose membranes for 48 hours at 37°C, then treated with gold nanoparticles (50 ppm and 100 ppm) and silver nanoparticles (50 ppm and 100 ppm) for another 48 hours. Antibacterial and antibiofilm activities were evaluated through cell density and SEM analysis. SEM revealed lower cell density, reduced biofilm formation, and altered cell morphology with rough, wrinkled surfaces after nanoparticle treatment. In conclusion, gold and silver nanoparticles exhibit antibacterial and antibiofilm properties, as observed in SEM analysis. SEM is a valuable tool for studying the antimicrobial effects of nano gold and silver on bacterial cell morphology and biofilm populations.
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CiteScore
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