继发性淋巴水肿小鼠模型淋巴再生的扫描电镜分析:初步研究

Kenji Hayashida, Ryohei Ogino, Shota Suda, Sho Yamakawa
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引用次数: 0

摘要

在包括淋巴疾病在内的炎症条件下,骨髓来源的髓样细胞通常表达淋巴内皮细胞(LEC)标记物,这些细胞被称为LEC祖细胞,它们通过与现有淋巴管融合而扩展淋巴管。然而,利用淋巴结构的三维图像对淋巴再生机制的研究是有限的。本研究采用扫描电镜(SEM)观察小鼠继发性淋巴水肿模型中淋巴管生成的三维结构。以C57BL/6J小鼠为模型,左后肢腹股沟区环形切口造模。术后第2、5、8天从淋巴水肿区取皮肤样本。为了通过扫描电镜分析确定淋巴管的位置,我们在一系列切片上检测了抗淋巴管内皮透明质酸受体1 (LYVE-1)的免疫反应性,并在扫描电镜观察时将其叠加。在第2天和第5天,球形细胞,可能是髓系细胞,附着并融合到lyve -1阳性的淋巴管壁上。第8天,球形细胞转化为弦状细胞,形成类似腔内柱的新淋巴管壁。我们的结果显示新形成的淋巴管壁延伸到管腔,提示肠套管性淋巴管生成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Scanning Electron Microscopy Analysis of Lymphatic Regeneration in a Secondary Lymphedema Mouse Model: A Preliminary Study
Under inflammatory conditions including lymphatic disorders, bone marrow-derived myeloid cells often express lymphatic endothelial cell (LEC) markers, and these cells are then called LEC progenitor cells, which extend lymphatic vessels by fusing with existing lymphatic vessels. However, studies on the mechanism of lymphatic regeneration using three-dimensional images of lymphatic structures are limited. In this study, scanning electron microscopy (SEM) was used to observe the three-dimensional structure of lymphangiogenesis in a mouse model of secondary lymphedema. The model was established in C57BL/6J mice via circumferential incision in the inguinal region of the left hind limb. Skin samples were obtained from the lymphedema region on days 2, 5, and 8 after surgery. To determine lymphatic vessel positions using SEM analysis, we detected anti-lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) immunoreactivity in serial sections and overlaid them during SEM observation. On days 2 and 5, spherical cells, probably myeloid cells, were attached and fused to the LYVE-1-positive lymphatic vessel walls. On day 8, spherical cells were converted to string-shaped cells, forming a new lymphatic vessel wall resembling an intraluminal pillar. Our results showed the newly formed lymphatic vessel wall extended into the lumen, suggesting intussusceptive lymphangiogenesis.
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