OPTIMASI DETEKSI GEN PADA Stelechocarpus burahol (Bl.) Hook.f. & Th. MENGGUNAKAN DIRECT KIR PCR

DNA isolation and purification in the conventional Polymerase Chain Reaction (PCR) process require reagents that are toxic, more costly and time consuming, and contamination. S. burahol leaves contain phenolics, flavonoids, and terpenoids which can interfere with DNA isolation. The use of direct PCR kits can detect genes without DNA extraction.The objective of study was to determinethe method of gene detection ofStelechocarpus buraholusing directPCRkit.In each location, one tree was taken as a source of leaf samples from Garut, Purwodadi Botanical Gardens, Kyai Langgeng Gardens, Yogyakarta Palace, Turi Sleman, Wanagama, Karanganyar, and South Kalimantan, except Bogor Botanical Gardens, two trees were taken. The primers used for the trials were ITS 1F primers and 4R primers. In the sequencing stage, PCR product samples of 40 -50 μl that showed positive results were detected by electrophoresis. The PCR product was measuredat ± 750 bp from ten samples. Direct PCR kits can be used for S. buraholgene detection, time and energy efficient, only requires a small amount of tissue, and reduces contamination due to DNA extraction. Direct PCR kits can be an effective method that can be utilized to detect target genesfor large populations.