A. K. Khalilah, M. Shuhaimi, M. Rosfarizan, A. Arbakariya, A. Yazid
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Optimization of fish gelatin-alginate-genipin as encapsulating matrices for probiotic application using FCCD-RSM
The optimal composition of fish gelatin-alginate-genipin to encapsulate Bifidobacterium pseudocatenulatum G4 was studied. The Face Central Composition Design-Response Surface Methodology (FCCD-RSM) was employed to determine the optimal ration of the matrices in order to obtain high in encapsulation yield (%) and beads strength. Results indicated that optimized matrices could increase the entrapped viable cells and beads strength. The optimal combination of encapsulating matrices was found to be 12.57 % for fish gelatin combined with 5 % alginate and 19.1 mM genipin. Verification experiment confirmed the prediction with low value error and gave indication a good performance prognosis of the optimal formulation.
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