人单核细胞和t细胞的电润湿和介质电泳无标记分离和分选

The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems Pub Date : 2013-04-07 DOI:10.1109/NEMS.2013.6559863

Cheng-Yeh Huang, M. Chiang, S. Fan, A. Ghaemmaghami, W. Hsu

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引用次数: 1

摘要

我们报道了一种基于电介质上电润湿(EWOD)和介电电泳(DEP)的无标记分离和分选方法，可以在液滴中操纵人类单核细胞(THP-1)和t细胞(Jurkat)系，而不影响细胞活力。除了分离细胞外，该装置还提供了一个平台来研究分离前细胞间的相互作用和迁移。

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Label-free separation and sorting of human monocytes and T-cells by electrowetting and dielectrophoresis

We report a label-free separation and sorting approach based on electrowetting-on-dielectric (EWOD) and dielectrophoresis (DEP) to manipulate human monocyte (THP-1) and T-cell (Jurkat) lines in droplets without any impact on cell viability. In addition to separating cells, this device provides a platform to study cell-cell interactions and migration before the separation.

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The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems

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