{"title":"用于资源有限地区现场部署的移动一体化疟疾分子诊断","authors":"G. Choi, Daniel Song, J. Miao, L. Cui, W. Guan","doi":"10.1109/HIC.2016.7797734","DOIUrl":null,"url":null,"abstract":"This paper presents a \"sample-in-answer-out\" molecular diagnostic system (AnyMDx) for blood-born malaria detection at the point of care, especially in resource-limiting settings. AnyMDx consists of a microfluidic reagent compact disk incorporating seamlessly integrated steps of DNA extraction, purification, elution, and amplification, as well as a mobile battery-powered analyzer. Low power thermal module and novel fluorescence sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP). The AnyMDx has an assay detection limit of ~0.6 parasites/μL against blood P. falciparum parasites, much lower than conventional microscope-based method (~50 parasites/μL) and immunoassay based RTD (~100 parasites/μL). AnyMDx is fully automated and thus is easily accessible to non-specialist with minimal molecular training. The sample-to-answer turnaround time for AnyMDx is less than 40 minutes.","PeriodicalId":333642,"journal":{"name":"2016 IEEE Healthcare Innovation Point-Of-Care Technologies Conference (HI-POCT)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas\",\"authors\":\"G. Choi, Daniel Song, J. Miao, L. Cui, W. Guan\",\"doi\":\"10.1109/HIC.2016.7797734\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This paper presents a \\\"sample-in-answer-out\\\" molecular diagnostic system (AnyMDx) for blood-born malaria detection at the point of care, especially in resource-limiting settings. AnyMDx consists of a microfluidic reagent compact disk incorporating seamlessly integrated steps of DNA extraction, purification, elution, and amplification, as well as a mobile battery-powered analyzer. Low power thermal module and novel fluorescence sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP). The AnyMDx has an assay detection limit of ~0.6 parasites/μL against blood P. falciparum parasites, much lower than conventional microscope-based method (~50 parasites/μL) and immunoassay based RTD (~100 parasites/μL). AnyMDx is fully automated and thus is easily accessible to non-specialist with minimal molecular training. The sample-to-answer turnaround time for AnyMDx is less than 40 minutes.\",\"PeriodicalId\":333642,\"journal\":{\"name\":\"2016 IEEE Healthcare Innovation Point-Of-Care Technologies Conference (HI-POCT)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2016 IEEE Healthcare Innovation Point-Of-Care Technologies Conference (HI-POCT)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/HIC.2016.7797734\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2016 IEEE Healthcare Innovation Point-Of-Care Technologies Conference (HI-POCT)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/HIC.2016.7797734","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas
This paper presents a "sample-in-answer-out" molecular diagnostic system (AnyMDx) for blood-born malaria detection at the point of care, especially in resource-limiting settings. AnyMDx consists of a microfluidic reagent compact disk incorporating seamlessly integrated steps of DNA extraction, purification, elution, and amplification, as well as a mobile battery-powered analyzer. Low power thermal module and novel fluorescence sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP). The AnyMDx has an assay detection limit of ~0.6 parasites/μL against blood P. falciparum parasites, much lower than conventional microscope-based method (~50 parasites/μL) and immunoassay based RTD (~100 parasites/μL). AnyMDx is fully automated and thus is easily accessible to non-specialist with minimal molecular training. The sample-to-answer turnaround time for AnyMDx is less than 40 minutes.