金针菇和香菇的体外培养

Nancy Mariel Casanova Palomeque, V. Bertolini, Leobardo Iracheta Donjuan
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引用次数: 0

摘要

本文介绍了从天南星科植物的叶片中无菌培养金针菇(Monstera acuminata Koch)和香菇(Monstera deliciosa Liebm)的基础,以及从幼芽茎盘中诱导金针菇离体器官发生的方法。采用不同的消毒方法对两种植物的成熟叶片和幼芽进行消毒,分别提取1 cm2的叶面外植体和约1 mm厚的茎盘。在无菌培养和器官发生诱导阶段,外植体在不同激素处理的半固体培养基中培养。用3%的次氯酸钠(NaClO)消毒20分钟,用含PPM的50% Murashige - Skoog培养基(1962)消毒,两种植物叶片外植体的氧化程度都较低。在添加PPM和不同消毒方案的培养基中,两种处理的美味乳杆菌外植体均成活,未出现污染,培养至49 d, 80%以上的外植体保持应答能力。培养35 d后,用Tween 20 +乙醇20% + 2.5% NaClO消毒,在添加1mg /l BAP、0.5 mg/l AIA和0.1 mg/l ANA的MS培养基上播种,可诱导出7个茎盘新芽。美味菌对体外条件的适应能力较好。在柳条生产中建立和诱导本地桤木的器官发生方面取得的进展,为当地种群的迁地保护奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro establishment of Monstera acuminata Koch and Monstera deliciosa Liebm
In this paper we present the bases for the aseptic establishment of Monstera acuminata Koch and Monstera deliciosa Liebm (Araceae) from leaves and the induction of in vitro organogenesis of M. acuminata K. from discs of stems of young shoots. Different disinfection protocols were applied to mature leaves and young shoots, from which foliar explants of 1 cm2 of both species were extracted, and stem disks approximately 1 mm of thick in M. acuminata K. The explants were established in semi-solid media with different hormonal treatments during the stage of aseptic establishment and induction of organogenesis. Disinfection with 3 % of sodium hypochlorite (NaClO) for 20 min and 50 % Murashige y Skoog medium (1962) with PPM, led to less oxidation in the leaf explants of both species. All the explants in the two treatments belonging to M. deliciosa, grown in medium added with PPM and different disinfection protocols, survived, did not present contamination and more than 80 % maintained answer’s capacity until 49 days of culture. After 35 days of culture, with disinfection in Tween 20 + Ethanol 20% + 2.5% NaClO, and sowing of the explants in the MS medium added with 1 mg/l of BAP, 0.5 mg/l of AIA and 0.1 mg/l of ANA were indueces seven new shoots of stem disks. M. deliciosa had better ability to adapt to in vitro conditions. Advances in the establishment and induction of organogenesis in native araceas for wicker production are the basis for ex situ conservation of local populations.
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