内生真菌的殖民能力和辣椒种子的生长能力

E. P. Ramdan, Efi Toding Tondok, Suryo Wiyono, S. H. Hidayat, W. Widodo
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引用次数: 0

摘要

内生真菌是生活在健康植物组织中而不引起疾病症状的真菌。辣椒内生真菌既可作为生物防治剂,又可作为生长促进剂,但其定殖尚未见报道。因此,本研究旨在确定内生真菌的定殖水平及其对辣椒幼苗生长的影响。以2.8 × 106 CFU mL-1的密度制备了8株内生真菌。然后接种内生真菌2次,第一次是浸泡种子,第二次是在播种后3周的辣椒幼苗上浇灌内生真菌悬浮液。在4周播种后的辣椒幼苗上重新分离内生真菌,测定其定植能力。辣椒种子在移植后保存4周,观察其生长情况。结果表明:内生真菌在辣椒根部的定殖率为26 ~ 60%,在茎基部的定殖率为20 ~ 40%,且定殖分布规律不同;此外,内生真菌定殖还能增加辣椒幼苗的茎高和根长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Kemampuan Kolonisasi Cendawan Endofit dan Peningkatan Pertumbuhan Bibit Cabai
Endophytic fungi are fungi that live in healthy plant tissues without causing disease symptoms. Endophytic fungi in chili have been tested as both biocontrol agents and growth promoters, but their colonization has not been reported. Therefore, this study aimed to determine the level of colonization of endophytic fungi and its effect on the growth of chili seedlings. A total of 8 endophytic fungi were prepared at a density of 2.8 × 106 CFU mL-1. Then the endophytic fungus was inoculated 2 times, first by soaking the seeds, and secondly by watering the endophytic fungus suspension on chili seedlings aged 3 weeks after sowing. Endophytic fungi were re-isolated on chili seedlings that were 4 weeks old after sowing on the roots and stems to determine their colonization ability. Chili seeds were then maintained for up to 4 weeks after transplanting to observe their growth. The results showed that the endophytic fungal colonization ranged from 26-60% on the chili root, while at the base of the stem it was 20-40% with a different pattern of colonization distribution. In addition, endophytic fungus colonization was also able to increase the shoot height and root length of chili seedlings.
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