Techniques of Microalga Culture Nannochloropsis oculata as a Natural Feed Source

Nannochloropsis oculata is a microalgae that acts as a natural food source in fisheries. The need for N. oculata can be met through progressive culture starting from laboratory scale culture to provide inoculant stock to mass culture using concrete tubs. The culture of N. oculata begins with the preparation and sterilization of culture media and equipment, fertilizer production, microalgae culture starting from the laboratory scale, intermediate scale, and mass scale, observing the density of microalgae and water quality. The lag phase of N. oculata occurs on the first and second days, the exponential growth phase lasts for four days, followed by a stationary phase that occurs on the seventh day. Rotifer contamination occurs in mass culture tanks. Production of  N. oculata can be carried out in bulk while still paying attention to every stage of culture preparation and microalgae culture activities as a standard operating procedure for microalgae culture to prevent contamination.   Keywords: progressive culture, microalgae, Nannochloropsis oculata