可可可可豆的总抗氧化物提取物(可可可种子碱)容量。L) Uv-Vis光谱测量方法

Agung Ari Chandra Wibawa
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引用次数: 1

摘要

抗氧化化合物是人体减少自由基所需要的化合物之一。抗氧化剂减少自由基的能力被称为抗氧化能力。本研究采用分光光度计紫外可见法测定可可豆甲醇提取物的总抗氧化能力。所得粗提物采用甲醇溶剂浸渍法提取。对可可豆甲醇提取物进行植物化学筛选试验,结果表明其含有黄酮类化合物和单宁类化合物。可可豆提取物的总抗氧化能力测试采用没食子酸的标准当量,在517 nm波长处显示出较强的抗氧化能力,可以降低DPPH自由基。测定了不同处理条件下,晒干可可豆和烘培可可豆的总抗氧化能力分别为27.730和42.454 mg/mL GAEAC。根据本研究结果,烘培可可豆乙醇提取物的总抗氧化能力最大,为42.454 mg/mL GAEAC。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Kapasitas Total Antioksidan Ekstrak Metanol Biji Kakao (Theobroma cacao. L) Dengan Metode Spektrofotometri Uv-Vis
Antioxidant compounds is one of compounds are needed by the body to reduce free radicals. The ability of antioxidants to reduce free radicals is called antioxidant capacity. The purpose of this study was to provide information on the total antioxidant capacity of the methanol extract of cocoa beans with the DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method using spectrophotometer uv-vis. The crude extract obtained was extracted using methanol solvent by maceration method. The results of the phytochemical screening test on the methanol extract of cocoa beans showed the presence of flavonoids and tannins. The total antioxidant capacity test of cocoa bean extract used the standard equivalent value of gallic acid which has been shown to have a strong antioxidant ability to reduce DPPH free radicals at a wavelength of 517 nm. The results of the measurement of total antioxidant capacity in the variation of treatment, namely sun-dried cocoa beans and oven cocoa beans were 27.730 and 42.454 mg/mL GAEAC. Based on the results of this study, it was found that the ethanol extract of oven cocoa beans had the greatest total antioxidant capacity, namely 42.454 mg/mL GAEAC.
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