褐藻(Padina pavonica, L.)的抗氧化活性阿尔及利亚地中海沿岸的提取物

Khadidja KERZABI-KANOUN, Nabila BELYAGOUBI-BENHAMMOU, Larbi BELYAGOUBI, Meriem BENMAHDJOUB, Ghania AISSAOUI, Wassila BENGHEDDA, Fawzia ATIK- BEKKARA
{"title":"褐藻(Padina pavonica, L.)的抗氧化活性阿尔及利亚地中海沿岸的提取物","authors":"Khadidja KERZABI-KANOUN, Nabila BELYAGOUBI-BENHAMMOU, Larbi BELYAGOUBI, Meriem BENMAHDJOUB, Ghania AISSAOUI, Wassila BENGHEDDA, Fawzia ATIK- BEKKARA","doi":"10.46325/jnpra.v1i02.15","DOIUrl":null,"url":null,"abstract":"The crude methanolic extract, ethyl acetate and butanolic fractions, tannins, saponins and polysaccharides of the marine brown alga, Padina pavonica, growing on the Algerian Mediterranean Coast were measured for antioxidant activity, using total antioxidant capacity (TAC), reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. Total phenolic, flavonoid and proanthocyanidin contents were determined. The total phenolic, proanthocyanidin and flavonoid contents of methanolic extract were about of 2.007 ± 0.104 mg GAE/g DM, 4.611 ± 0.346 mg CE/g DM and 1.132 ± 0.091 mg CE/g DM, respectively. The total antioxidant capacity was higher (4.365 ± 0.452 mg AAE/g DM) in crude methanolic extract. For the other two tests, the tannins and ethyl acetate fraction presented high activities compared to the other extracts with EC50 about 1.430 ± 0.000 and 1.615 ± 0.009 mg/mL), respectively for the reducing power and 5.718 ± 1.111 and 5.848 ± 0.186 mg/mL for the DPPH radical scavenging. The seaweed extracts displayed moderate antioxidant activity compared to ascorbic acid. \nREFERENCES: \nAidi Wannes, W., Mhamdi, B., Sriti, J., Ben Jemia, M., Ouchikh, O., Hamdaoui, G., Kchouk,M.E., & Marzouk, B. (2010). Antioxidant activities of the essential oils and methanol extracts from myrtle (Myrtus communis var. italica L.) leaf stem and flower. Food andChemistry Toxicology, 48 (5), 1362–1370.Applebaum, S.W., Marco, S., & Birk, Y. (1969). Saponins as possible factors of resistance oflegume seeds to the attack of insects. Journal of Agricultural and Food Chemistry, 17(3), 618–622.Bekkara, F., Jay, M., Viricel, M.R., & Rome, S. (1998). Distribution of phenolic compoundswithin seed and seedlings of tow Vicia faba cvs differing in their seed tannin content,and study of to their seed and root phenolic exudation. Plant and Soil, 203 (1), 27–36.Ben Said, R., El Abed, A., & Romdhane, M. S. (2002). Etude d’une Population de l’alguebrune Padina pavonica (L) Lamouroux A Cap Zebib (Nord De La Tunisie). Bulletin del'Institut National Scientifique et Technique d'Oceanographie et de Peche deSalammbo, 29, 95–103.Chbani, A., Mawlawi, H., & Eltahiri, S. (2011). Antibacterial activity of brown seaweedextracts of Padina pavonica from the Mediterranean coast of Lebanon. Phytothérapie,9, 283–286.Chbani, A., Mawlawi, H., & Zaouk, L. (2013). Evaluation of brown seaweed (Padinapavonica) as biostimulant of plant growth and development. African Journal ofAgricultural Research, 8 (13), 1155–1165.Demirel, Z., Yildirim, Z.D., Tuney, I., Kesici, K., & Sukatar, A. (2012). Biochemical analysisof some brown seaweeds from Aegean Sea. Botanica Serbica, 36, 91-95.Fischer, W., Scheider, M., & Bauchot, M.L. (1987). Fiches FAO d'identification des espècespour les besoins de la pêche. Méditerranée et Mer noire; Zone de pêche 37. [Speciesidentification sheets FAO for fishery purposes. Mediterranean and black sea; fishing area37].Vol.1. Food and Agriculture Organisation of the United Nations, Rome. p. 758.Indu, H., & Seenivasan, R. (2013). In vitro antioxidant activity of selected seaweeds fromsoutheast coast of India, International Journal of Pharmacy and PharmaceuticalSciences, 5(2), 474–484.Ismail-Ben Ali, A., Ktari, L., Boudabbous, A., & El Bour, M. (2010). Seasonal variation ofantibacterial activity of the brown alga Padina pavonica (L) thivy collected fromNorthern coast of Tunisia. Bulletin de l'Institut National Scientifique et Techniqued'Oceanographie et de Peche de Salammbo, 37, 111–116.Julkunen-Titto, R. (1985). Phenolic constituents in the leaves of northern willows: methodsfor the analysis of certain phenolics. Journal of Agricultural and Food Chemistry, 33(2), 213–217.Kamenarska, Z., Gasic, M. J., Zlatovi, M., Razovic, A., Sladic, D., Kljajic, Z., Stefanov, K.,Seizova, K., Najdenski, H., Kujumgieva, A., Tsvetkova, I., & Popo, S. (2002).Chemical composition of the brown Alga Padina pavonia (L.) Gaill. from the AdriaticSea. Botanica Marina, 45, 339–345.Kokabi, M., Yousefzadi, M., Ali ahmadi, A., Feghhi, M.A., & Keshavarz, M. (2013).Antioxidant Activity of Extracts of Selected Algae from the Persian Gulf, Iran. Journalof the Persian Gulf (Marine Science), 4(6), 45–50.Ksouri, J., Elferjani, H., & Mensi, F. (2008). Estimation du stock naturel de l’algue brunePadina pavonica (L.) Thivy en Tunisie septentrionale (Cap Zebib). Bulletin de l'InstitutNational Scientifique et Technique d'Oceanographie et de Peche de Salammbo, 35, 57–60.Ktari, L., & Guyot, M. (1999). A cytotoxic oxysterol from the marine alga Padina pavonica(L.) Thivy. Journal of Applied Phycology, 11 (6), 511–513.Ktari, L., Longeon, A., & Guyot, M. (2001). Biological activities of marine algae fromTunisian coasts. Journal de Recherche Océanographique, 26, 37–41. \n Kumar Chandini, S., Ganesan, P., & Bhaskar, N. (2008). In vitro antioxidant activities ofthree selected brown seaweeds. Food Chemistry, 107, 707–713.Naja, K., Mawlawi, H., & Chbani, A. (2012). Antioxidant and Antifungal activities of PadinaPavonica and SargassumVulgarefrom the Lebanese Mediterranean Coast. Advances inEnvironmental Biology, 6(1), 42–48.Omezzine, F., Haouala, R., El Ayeb, A., & Boughanmi, N. (2009). Allelopathic andantifungal potentialities of Padina pavonica (L.) extract. Journal of Plant Breeding andCrop Science, 1(4), 194–203.Oyaizu, M. (1986). Studies one products of browning reaction prepared from glucose amine.Japanese Journal of Nutrition, 44 (6), 307–315.Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidantcapacity through the formation of a phosphomolybdenum complex: specific applicationto the determination of vitamin E. Analytical Biochemistry, 269 (2), 337–341.Sanchez-Moreno, C., Larrauri, J.A., & Saura-Calixto, F. (1998). A procedure to measure theantiradical efficiency of polyphenols. Journal of the Science of Food and Agriculture,76 (2), 270–276.Sari, A., & Tuzen, M. (2009). Equilibrium, thermodynamic and kinetic studies on aluminumbiosorption from aqueous solution by brown algae (Padina pavonica) biomass.Journal of Hazardous Materials, 171, 973–979Singleton, C.P., & Rossi, J.A. (1965). Colorimetry of Total Phenolics withPhosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enolology andViticulture, 16 (3), 144–158.Teodosio Melo, K.R., Gomes Camara., R.B., Queiroz, M.F., Jacome Vidal, A.A., MachadoLima, K.R., Melo-Silveira, R.F., Almeida-Lima, J., & Oliveira Rocha, H.A. (2013).Evaluation of Sulfated Polysaccharides from the Brown Seaweed Dictyopteris Justii asAntioxidant Agents and as .Inhibitors of the Formation of Calcium Oxalate Crystals.Molecules, 18, 14543–14563Wijesekara, I., Pangestutia, R., & Kima, S.K. (2011). Biological activities and potentialhealth benefits of sulfated polysaccharides derived from marine algae. CarbohydratePolymers, 84, 14–21Zhang, S.Y., Zheng, C.G., Yan, X.Y., & Tian, W.X. (2008). Low concentration of condensedtannins from catechu significantly inhibits fatty acid synthase and growth of MCF-7concealments. Biochemical and Biophysical Research Communications, 371 (4), 654–658.Zhenfei, P., Min, L., Zhexiang, F., Jiulin, W., & Qiqing Z. (2012). Composition andcytotoxicity of a novel polysaccharide from brown alga (Laminaria japonica).Carbohydrate Polymers, 89, 1022– 1026.Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contentin mulberry and to their scanenging effects one superoxide radicals. Food Chemistry,64 (4), 555–559.","PeriodicalId":286353,"journal":{"name":"Journal of Natural Product Research and Applications","volume":"3 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Antioxidant Activity of Brown Seaweed (Padina pavonica (L.) Extracts From the Algerian Mediterranean Coast\",\"authors\":\"Khadidja KERZABI-KANOUN, Nabila BELYAGOUBI-BENHAMMOU, Larbi BELYAGOUBI, Meriem BENMAHDJOUB, Ghania AISSAOUI, Wassila BENGHEDDA, Fawzia ATIK- BEKKARA\",\"doi\":\"10.46325/jnpra.v1i02.15\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The crude methanolic extract, ethyl acetate and butanolic fractions, tannins, saponins and polysaccharides of the marine brown alga, Padina pavonica, growing on the Algerian Mediterranean Coast were measured for antioxidant activity, using total antioxidant capacity (TAC), reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. Total phenolic, flavonoid and proanthocyanidin contents were determined. The total phenolic, proanthocyanidin and flavonoid contents of methanolic extract were about of 2.007 ± 0.104 mg GAE/g DM, 4.611 ± 0.346 mg CE/g DM and 1.132 ± 0.091 mg CE/g DM, respectively. The total antioxidant capacity was higher (4.365 ± 0.452 mg AAE/g DM) in crude methanolic extract. For the other two tests, the tannins and ethyl acetate fraction presented high activities compared to the other extracts with EC50 about 1.430 ± 0.000 and 1.615 ± 0.009 mg/mL), respectively for the reducing power and 5.718 ± 1.111 and 5.848 ± 0.186 mg/mL for the DPPH radical scavenging. The seaweed extracts displayed moderate antioxidant activity compared to ascorbic acid. \\nREFERENCES: \\nAidi Wannes, W., Mhamdi, B., Sriti, J., Ben Jemia, M., Ouchikh, O., Hamdaoui, G., Kchouk,M.E., & Marzouk, B. (2010). Antioxidant activities of the essential oils and methanol extracts from myrtle (Myrtus communis var. italica L.) leaf stem and flower. Food andChemistry Toxicology, 48 (5), 1362–1370.Applebaum, S.W., Marco, S., & Birk, Y. (1969). Saponins as possible factors of resistance oflegume seeds to the attack of insects. Journal of Agricultural and Food Chemistry, 17(3), 618–622.Bekkara, F., Jay, M., Viricel, M.R., & Rome, S. (1998). Distribution of phenolic compoundswithin seed and seedlings of tow Vicia faba cvs differing in their seed tannin content,and study of to their seed and root phenolic exudation. Plant and Soil, 203 (1), 27–36.Ben Said, R., El Abed, A., & Romdhane, M. S. (2002). Etude d’une Population de l’alguebrune Padina pavonica (L) Lamouroux A Cap Zebib (Nord De La Tunisie). Bulletin del'Institut National Scientifique et Technique d'Oceanographie et de Peche deSalammbo, 29, 95–103.Chbani, A., Mawlawi, H., & Eltahiri, S. (2011). Antibacterial activity of brown seaweedextracts of Padina pavonica from the Mediterranean coast of Lebanon. Phytothérapie,9, 283–286.Chbani, A., Mawlawi, H., & Zaouk, L. (2013). Evaluation of brown seaweed (Padinapavonica) as biostimulant of plant growth and development. African Journal ofAgricultural Research, 8 (13), 1155–1165.Demirel, Z., Yildirim, Z.D., Tuney, I., Kesici, K., & Sukatar, A. (2012). Biochemical analysisof some brown seaweeds from Aegean Sea. Botanica Serbica, 36, 91-95.Fischer, W., Scheider, M., & Bauchot, M.L. (1987). Fiches FAO d'identification des espècespour les besoins de la pêche. Méditerranée et Mer noire; Zone de pêche 37. [Speciesidentification sheets FAO for fishery purposes. Mediterranean and black sea; fishing area37].Vol.1. Food and Agriculture Organisation of the United Nations, Rome. p. 758.Indu, H., & Seenivasan, R. (2013). In vitro antioxidant activity of selected seaweeds fromsoutheast coast of India, International Journal of Pharmacy and PharmaceuticalSciences, 5(2), 474–484.Ismail-Ben Ali, A., Ktari, L., Boudabbous, A., & El Bour, M. (2010). Seasonal variation ofantibacterial activity of the brown alga Padina pavonica (L) thivy collected fromNorthern coast of Tunisia. Bulletin de l'Institut National Scientifique et Techniqued'Oceanographie et de Peche de Salammbo, 37, 111–116.Julkunen-Titto, R. (1985). Phenolic constituents in the leaves of northern willows: methodsfor the analysis of certain phenolics. Journal of Agricultural and Food Chemistry, 33(2), 213–217.Kamenarska, Z., Gasic, M. J., Zlatovi, M., Razovic, A., Sladic, D., Kljajic, Z., Stefanov, K.,Seizova, K., Najdenski, H., Kujumgieva, A., Tsvetkova, I., & Popo, S. (2002).Chemical composition of the brown Alga Padina pavonia (L.) Gaill. from the AdriaticSea. Botanica Marina, 45, 339–345.Kokabi, M., Yousefzadi, M., Ali ahmadi, A., Feghhi, M.A., & Keshavarz, M. (2013).Antioxidant Activity of Extracts of Selected Algae from the Persian Gulf, Iran. Journalof the Persian Gulf (Marine Science), 4(6), 45–50.Ksouri, J., Elferjani, H., & Mensi, F. (2008). Estimation du stock naturel de l’algue brunePadina pavonica (L.) Thivy en Tunisie septentrionale (Cap Zebib). Bulletin de l'InstitutNational Scientifique et Technique d'Oceanographie et de Peche de Salammbo, 35, 57–60.Ktari, L., & Guyot, M. (1999). A cytotoxic oxysterol from the marine alga Padina pavonica(L.) Thivy. Journal of Applied Phycology, 11 (6), 511–513.Ktari, L., Longeon, A., & Guyot, M. (2001). Biological activities of marine algae fromTunisian coasts. Journal de Recherche Océanographique, 26, 37–41. \\n Kumar Chandini, S., Ganesan, P., & Bhaskar, N. (2008). In vitro antioxidant activities ofthree selected brown seaweeds. Food Chemistry, 107, 707–713.Naja, K., Mawlawi, H., & Chbani, A. (2012). Antioxidant and Antifungal activities of PadinaPavonica and SargassumVulgarefrom the Lebanese Mediterranean Coast. Advances inEnvironmental Biology, 6(1), 42–48.Omezzine, F., Haouala, R., El Ayeb, A., & Boughanmi, N. (2009). Allelopathic andantifungal potentialities of Padina pavonica (L.) extract. Journal of Plant Breeding andCrop Science, 1(4), 194–203.Oyaizu, M. (1986). Studies one products of browning reaction prepared from glucose amine.Japanese Journal of Nutrition, 44 (6), 307–315.Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidantcapacity through the formation of a phosphomolybdenum complex: specific applicationto the determination of vitamin E. Analytical Biochemistry, 269 (2), 337–341.Sanchez-Moreno, C., Larrauri, J.A., & Saura-Calixto, F. (1998). A procedure to measure theantiradical efficiency of polyphenols. Journal of the Science of Food and Agriculture,76 (2), 270–276.Sari, A., & Tuzen, M. (2009). Equilibrium, thermodynamic and kinetic studies on aluminumbiosorption from aqueous solution by brown algae (Padina pavonica) biomass.Journal of Hazardous Materials, 171, 973–979Singleton, C.P., & Rossi, J.A. (1965). Colorimetry of Total Phenolics withPhosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enolology andViticulture, 16 (3), 144–158.Teodosio Melo, K.R., Gomes Camara., R.B., Queiroz, M.F., Jacome Vidal, A.A., MachadoLima, K.R., Melo-Silveira, R.F., Almeida-Lima, J., & Oliveira Rocha, H.A. (2013).Evaluation of Sulfated Polysaccharides from the Brown Seaweed Dictyopteris Justii asAntioxidant Agents and as .Inhibitors of the Formation of Calcium Oxalate Crystals.Molecules, 18, 14543–14563Wijesekara, I., Pangestutia, R., & Kima, S.K. (2011). Biological activities and potentialhealth benefits of sulfated polysaccharides derived from marine algae. CarbohydratePolymers, 84, 14–21Zhang, S.Y., Zheng, C.G., Yan, X.Y., & Tian, W.X. (2008). Low concentration of condensedtannins from catechu significantly inhibits fatty acid synthase and growth of MCF-7concealments. Biochemical and Biophysical Research Communications, 371 (4), 654–658.Zhenfei, P., Min, L., Zhexiang, F., Jiulin, W., & Qiqing Z. (2012). Composition andcytotoxicity of a novel polysaccharide from brown alga (Laminaria japonica).Carbohydrate Polymers, 89, 1022– 1026.Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contentin mulberry and to their scanenging effects one superoxide radicals. 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引用次数: 2

摘要

产自黎巴嫩地中海沿岸的凤尾菜和凤尾菜的抗氧化和抗真菌活性。环境生物学进展,6(1),42-48。Omezzine, F., Haouala, R., El Ayeb, A., & Boughanmi, N.(2009)。金莲花提取物的化感作用和抗真菌作用。植物育种与作物科学,1(4),194-203。Oyaizu M.(1986)。研究了葡萄糖胺褐变反应的一种产物。营养学报,44(6),307-315。Prieto, P., Pineda, M.和Aguilar, M.(1999)。通过形成磷钼络合物的分光光度法测定抗氧化能力:在维生素e测定中的特殊应用。Sanchez-Moreno, C, Larrauri, j.a.和Saura-Calixto, F.(1998)。一种测量多酚抗自由基效率的方法。中国农业科学,2016(2),391 - 391。Sari, A., & Tuzen, M.(2009)。褐藻(Padina pavonica)生物质吸附铝的平衡、热力学和动力学研究中国环境科学学报,1997,17(3):973 - 979。磷钼-磷钨酸试剂测定总酚类物质的比色法。中国酿酒科学,2016(3),344 - 344。特奥多西奥·梅洛,k.r.,戈麦斯·卡马拉。, r.b., Queiroz, m.f., Jacome Vidal, a.a., MachadoLima, k.r., Melo-Silveira, r.f., Almeida-Lima, J.,和Oliveira Rocha, H.A.(2013)。褐藻硫酸酸化多糖作为抗氧化剂和草酸钙晶体形成抑制剂的评价。魏杰卡拉,李建平,李建平,等(2011).分子工程学报,18,14543 - 14563海藻硫酸酸化多糖的生物活性及其潜在的健康益处。张素英,郑春光,闫晓燕,田文祥(2008).碳水化合物高分子,84,14 - 21儿茶中低浓度的浓缩单宁显著抑制脂肪酸合成酶和mcf -7隐蔽物的生长。中国生物工程学报,2016,31(4),344 - 344。彭振飞,李敏,何振祥,冯振林,王久林,张启清(2012)。一种新型褐藻(海带)多糖的组成及细胞毒性。碳水化合物高分子,89,1022 - 1026。J.智深,T.孟成,W.建明(1999)。桑树中类黄酮含量的测定及其对超氧自由基的扫描作用。食品化学,64(4),555-559。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antioxidant Activity of Brown Seaweed (Padina pavonica (L.) Extracts From the Algerian Mediterranean Coast
The crude methanolic extract, ethyl acetate and butanolic fractions, tannins, saponins and polysaccharides of the marine brown alga, Padina pavonica, growing on the Algerian Mediterranean Coast were measured for antioxidant activity, using total antioxidant capacity (TAC), reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. Total phenolic, flavonoid and proanthocyanidin contents were determined. The total phenolic, proanthocyanidin and flavonoid contents of methanolic extract were about of 2.007 ± 0.104 mg GAE/g DM, 4.611 ± 0.346 mg CE/g DM and 1.132 ± 0.091 mg CE/g DM, respectively. The total antioxidant capacity was higher (4.365 ± 0.452 mg AAE/g DM) in crude methanolic extract. For the other two tests, the tannins and ethyl acetate fraction presented high activities compared to the other extracts with EC50 about 1.430 ± 0.000 and 1.615 ± 0.009 mg/mL), respectively for the reducing power and 5.718 ± 1.111 and 5.848 ± 0.186 mg/mL for the DPPH radical scavenging. The seaweed extracts displayed moderate antioxidant activity compared to ascorbic acid. REFERENCES: Aidi Wannes, W., Mhamdi, B., Sriti, J., Ben Jemia, M., Ouchikh, O., Hamdaoui, G., Kchouk,M.E., & Marzouk, B. (2010). Antioxidant activities of the essential oils and methanol extracts from myrtle (Myrtus communis var. italica L.) leaf stem and flower. Food andChemistry Toxicology, 48 (5), 1362–1370.Applebaum, S.W., Marco, S., & Birk, Y. (1969). Saponins as possible factors of resistance oflegume seeds to the attack of insects. Journal of Agricultural and Food Chemistry, 17(3), 618–622.Bekkara, F., Jay, M., Viricel, M.R., & Rome, S. (1998). Distribution of phenolic compoundswithin seed and seedlings of tow Vicia faba cvs differing in their seed tannin content,and study of to their seed and root phenolic exudation. Plant and Soil, 203 (1), 27–36.Ben Said, R., El Abed, A., & Romdhane, M. S. (2002). Etude d’une Population de l’alguebrune Padina pavonica (L) Lamouroux A Cap Zebib (Nord De La Tunisie). Bulletin del'Institut National Scientifique et Technique d'Oceanographie et de Peche deSalammbo, 29, 95–103.Chbani, A., Mawlawi, H., & Eltahiri, S. (2011). Antibacterial activity of brown seaweedextracts of Padina pavonica from the Mediterranean coast of Lebanon. Phytothérapie,9, 283–286.Chbani, A., Mawlawi, H., & Zaouk, L. (2013). Evaluation of brown seaweed (Padinapavonica) as biostimulant of plant growth and development. African Journal ofAgricultural Research, 8 (13), 1155–1165.Demirel, Z., Yildirim, Z.D., Tuney, I., Kesici, K., & Sukatar, A. (2012). Biochemical analysisof some brown seaweeds from Aegean Sea. Botanica Serbica, 36, 91-95.Fischer, W., Scheider, M., & Bauchot, M.L. (1987). Fiches FAO d'identification des espècespour les besoins de la pêche. Méditerranée et Mer noire; Zone de pêche 37. [Speciesidentification sheets FAO for fishery purposes. Mediterranean and black sea; fishing area37].Vol.1. Food and Agriculture Organisation of the United Nations, Rome. p. 758.Indu, H., & Seenivasan, R. (2013). In vitro antioxidant activity of selected seaweeds fromsoutheast coast of India, International Journal of Pharmacy and PharmaceuticalSciences, 5(2), 474–484.Ismail-Ben Ali, A., Ktari, L., Boudabbous, A., & El Bour, M. (2010). Seasonal variation ofantibacterial activity of the brown alga Padina pavonica (L) thivy collected fromNorthern coast of Tunisia. Bulletin de l'Institut National Scientifique et Techniqued'Oceanographie et de Peche de Salammbo, 37, 111–116.Julkunen-Titto, R. (1985). Phenolic constituents in the leaves of northern willows: methodsfor the analysis of certain phenolics. Journal of Agricultural and Food Chemistry, 33(2), 213–217.Kamenarska, Z., Gasic, M. J., Zlatovi, M., Razovic, A., Sladic, D., Kljajic, Z., Stefanov, K.,Seizova, K., Najdenski, H., Kujumgieva, A., Tsvetkova, I., & Popo, S. (2002).Chemical composition of the brown Alga Padina pavonia (L.) Gaill. from the AdriaticSea. Botanica Marina, 45, 339–345.Kokabi, M., Yousefzadi, M., Ali ahmadi, A., Feghhi, M.A., & Keshavarz, M. (2013).Antioxidant Activity of Extracts of Selected Algae from the Persian Gulf, Iran. Journalof the Persian Gulf (Marine Science), 4(6), 45–50.Ksouri, J., Elferjani, H., & Mensi, F. (2008). Estimation du stock naturel de l’algue brunePadina pavonica (L.) Thivy en Tunisie septentrionale (Cap Zebib). Bulletin de l'InstitutNational Scientifique et Technique d'Oceanographie et de Peche de Salammbo, 35, 57–60.Ktari, L., & Guyot, M. (1999). 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