微液滴中的单分子检测

M. Barnes, W. Whitten, J. Ramsey
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引用次数: 0

摘要

具有高信噪比的凝聚态单分子检测对于检测荧光标记(如DNA测序1、荧光免疫测定2或水文学)的应用非常重要此外,观测辐射场与孤立分子的相互作用所特有的光物理现象,如光子反聚束4,依赖于具有如此高的灵敏度。正如Moerner5和Orrit的实验所证明的那样,在低温下探测固体宿主中的“客体”分子可以在单分子水平上获得非常高的灵敏度。然而,需要单分子检测的实际应用通常需要在室温下对液相溶液进行测量。这些要求提出了新的问题,如溶剂依赖的量子产率,有限的光化学寿命(在荧光不可逆漂白之前发生的吸收-发射循环的次数),以及溶剂拉曼散射和荧光。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Single-Molecule Detection in Microdroplets
Detection of single molecules in condensed phase with high signal-to-noise ratios is important for applications which involve detection of fluorescent tags such as DNA sequencing1, fluorescence immunoassay2, or hydrology.3 In addition, observation of photophysical phenomena unique to the interaction of a radiation field with an isolatedmolecule, such as photon antibunching,4 depends upon having such high sensitivity. As demonstrated by the experiments of Moerner5 and Orrit,6 very high sensitivity at the single molecule level can be achieved by probing "guest" molecules in solid hosts at cryogenic temperatures. However, practical applications requiring single molecule detection usually require measurements on liquid phase solutions at room temperature. These demands pose new problems such as solvent dependent quantum yield, finite photochemical lifetime (the number of absorption-emission cycles which occur before irreversible bleaching of fluorescence), and solvent Raman scattering and fluorescence.
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