Active 3D fluorescence imaging based on holography

3D fluorescence imaging based on incoherent digital holography is very useful in the biological field. We have proposed a common-path and off-axis incoherent digital holography using a diffraction grating embedded with a lens to make the self-interference pattern. Based on the observation of the fluorescence image, active illumination of the holographic method is more efficient to avoid unwanted exposure to another area. We present the feasibility experiment to show the potential characteristics of our proposed system.