{"title":"利用市售单克隆抗体对头发样本ABO血型进行吸收-洗脱试验的最佳洗脱温度和时间","authors":"T. Ohmori, Hajime Sato","doi":"10.3408/JASTI.6.49","DOIUrl":null,"url":null,"abstract":"The optimum elution temperature and optimum elution time of absorption-elution test for ABO blood-typing from hair samples using commercially available monoclonal antibody were examined. Elution temperature was examined in the range of 51-57°C and elution time was examined in the range of 2-15 min. The eluting antibodies were semi-quantitatively analyzed by ELISA using synthetic blood group antigens. The optimum elution temperature and optimum elution time were determined for each monoclonal antibody. The procedure of absorption-elution method for hair samples was improved and the improved procedure was evaluated by a blind test. The improved procedure of the absorption-elution test gave a satisfactory result.","PeriodicalId":134327,"journal":{"name":"Japanese Journal of Science and Technology for Identification","volume":"260 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"The Optimum Elution Temperature and Time in Absorption-Elution Test Using Commercially Available Monoclonal Antibodies for ABO Blood-Typing from Hair Samples\",\"authors\":\"T. Ohmori, Hajime Sato\",\"doi\":\"10.3408/JASTI.6.49\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The optimum elution temperature and optimum elution time of absorption-elution test for ABO blood-typing from hair samples using commercially available monoclonal antibody were examined. Elution temperature was examined in the range of 51-57°C and elution time was examined in the range of 2-15 min. The eluting antibodies were semi-quantitatively analyzed by ELISA using synthetic blood group antigens. The optimum elution temperature and optimum elution time were determined for each monoclonal antibody. The procedure of absorption-elution method for hair samples was improved and the improved procedure was evaluated by a blind test. The improved procedure of the absorption-elution test gave a satisfactory result.\",\"PeriodicalId\":134327,\"journal\":{\"name\":\"Japanese Journal of Science and Technology for Identification\",\"volume\":\"260 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese Journal of Science and Technology for Identification\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3408/JASTI.6.49\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Science and Technology for Identification","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3408/JASTI.6.49","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The Optimum Elution Temperature and Time in Absorption-Elution Test Using Commercially Available Monoclonal Antibodies for ABO Blood-Typing from Hair Samples
The optimum elution temperature and optimum elution time of absorption-elution test for ABO blood-typing from hair samples using commercially available monoclonal antibody were examined. Elution temperature was examined in the range of 51-57°C and elution time was examined in the range of 2-15 min. The eluting antibodies were semi-quantitatively analyzed by ELISA using synthetic blood group antigens. The optimum elution temperature and optimum elution time were determined for each monoclonal antibody. The procedure of absorption-elution method for hair samples was improved and the improved procedure was evaluated by a blind test. The improved procedure of the absorption-elution test gave a satisfactory result.
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