Ai-Dong Sun, Yue Zhu, Yu-long Wei, Jia-ying Zhang, Nan Zhang, Yu-qing Lei, Zhilin Gan, Sift Desk Journals Open Access Journals
{"title":"采用NMR、LC-IT-TOF/MS/MS和MALDI-TOF质谱法分离鉴定黑檀原花青素","authors":"Ai-Dong Sun, Yue Zhu, Yu-long Wei, Jia-ying Zhang, Nan Zhang, Yu-qing Lei, Zhilin Gan, Sift Desk Journals Open Access Journals","doi":"10.25177/JFST.4.1.RA.444","DOIUrl":null,"url":null,"abstract":"Aronia melanocarpa berries procyanidins (Amps) were divided in three fractions: ethyl acetate phase extract of Amps (E-Amps), aqueous phase extract of Amps (W-Amps), and methanol phase extract of Amps (M-Amps). Flavan-3-ol monomers and oligomeric procyanidins were isolated from E-Amps using Sephadex LH-20 column chromatography and reversed-phase preparative HPLC. H and C NMR analyses indicated five flavan-3-ol monomers and oligomeric procyanidins: (+)catechin, (‒)epicatechin, procyanidin B2 [EC-(4β→8)-EC], procyanidin B1 [EC-(4β→8)-C], and procyanidin C1 [EC-(4β→8)-EC-(4β→8)-EC]. MALDI-TOF-MS analysis demonstrated that the structure units of polymeric Amps, which were linked by B-type bonds, contained only catechin and epicatechin. In addition, the degree of polymerization was from pentamer to twenty-two polymer. Five flavan-3-ol monomers and oligomeric procyanidins had strong antioxidant activities. The DPPH and ABTS free-radical scavenging capacities followed the order: flavan-3-ol monomers > dimeric procyanidins > trimeric procyanidins > ascorbic acid. However, FRAP values increased with a degree of polymerization.","PeriodicalId":269546,"journal":{"name":"SDRP Journal of Food Science & Technology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"Isolation and Identification of procyanidins in Aronia Melanocarpa Using NMR, LC-IT-TOF/MS/MS and MALDI-TOF MS\",\"authors\":\"Ai-Dong Sun, Yue Zhu, Yu-long Wei, Jia-ying Zhang, Nan Zhang, Yu-qing Lei, Zhilin Gan, Sift Desk Journals Open Access Journals\",\"doi\":\"10.25177/JFST.4.1.RA.444\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aronia melanocarpa berries procyanidins (Amps) were divided in three fractions: ethyl acetate phase extract of Amps (E-Amps), aqueous phase extract of Amps (W-Amps), and methanol phase extract of Amps (M-Amps). Flavan-3-ol monomers and oligomeric procyanidins were isolated from E-Amps using Sephadex LH-20 column chromatography and reversed-phase preparative HPLC. H and C NMR analyses indicated five flavan-3-ol monomers and oligomeric procyanidins: (+)catechin, (‒)epicatechin, procyanidin B2 [EC-(4β→8)-EC], procyanidin B1 [EC-(4β→8)-C], and procyanidin C1 [EC-(4β→8)-EC-(4β→8)-EC]. MALDI-TOF-MS analysis demonstrated that the structure units of polymeric Amps, which were linked by B-type bonds, contained only catechin and epicatechin. In addition, the degree of polymerization was from pentamer to twenty-two polymer. Five flavan-3-ol monomers and oligomeric procyanidins had strong antioxidant activities. The DPPH and ABTS free-radical scavenging capacities followed the order: flavan-3-ol monomers > dimeric procyanidins > trimeric procyanidins > ascorbic acid. However, FRAP values increased with a degree of polymerization.\",\"PeriodicalId\":269546,\"journal\":{\"name\":\"SDRP Journal of Food Science & Technology\",\"volume\":\"3 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-02-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"SDRP Journal of Food Science & Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.25177/JFST.4.1.RA.444\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"SDRP Journal of Food Science & Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25177/JFST.4.1.RA.444","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Isolation and Identification of procyanidins in Aronia Melanocarpa Using NMR, LC-IT-TOF/MS/MS and MALDI-TOF MS
Aronia melanocarpa berries procyanidins (Amps) were divided in three fractions: ethyl acetate phase extract of Amps (E-Amps), aqueous phase extract of Amps (W-Amps), and methanol phase extract of Amps (M-Amps). Flavan-3-ol monomers and oligomeric procyanidins were isolated from E-Amps using Sephadex LH-20 column chromatography and reversed-phase preparative HPLC. H and C NMR analyses indicated five flavan-3-ol monomers and oligomeric procyanidins: (+)catechin, (‒)epicatechin, procyanidin B2 [EC-(4β→8)-EC], procyanidin B1 [EC-(4β→8)-C], and procyanidin C1 [EC-(4β→8)-EC-(4β→8)-EC]. MALDI-TOF-MS analysis demonstrated that the structure units of polymeric Amps, which were linked by B-type bonds, contained only catechin and epicatechin. In addition, the degree of polymerization was from pentamer to twenty-two polymer. Five flavan-3-ol monomers and oligomeric procyanidins had strong antioxidant activities. The DPPH and ABTS free-radical scavenging capacities followed the order: flavan-3-ol monomers > dimeric procyanidins > trimeric procyanidins > ascorbic acid. However, FRAP values increased with a degree of polymerization.
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