{"title":"BAKDING DNA BURUNG ELANG (FAMILI ACCIPITRIDA) DI INDONESIA","authors":"M. Zein","doi":"10.14203/BERITABIOLOGI.V17I2.3108","DOIUrl":null,"url":null,"abstract":"The cytochrome c oxidase subunit I (COI) gene is a reprensentative of all the protein-coding genes of the mitochondrial DNA genome that has been widely used as an animal species identification tool. In this study, 86 sequences of DNA barcodes of members of the family Accipitridae in Indonesia including Nisaetus bartelsi, Nisaetus cirrhatus, Haliaeetus leucogaster, Spilornis cheela, Haliastur indus, and 11 sequences from Genbank were examined. Each species was confirmed through the Basic Local Alignment Search Tool (BLAST). The construction of phylogeny trees based on COI gene sequences was performed by the Neighbors-joining method where the calculation of the genetic distance matrix with the Kimura 2-parameter model was implemented in pairwise distance calculation in the Mega version 6.05 programe. The results of the analysis showed that the divergence within species ranged from 0 to 0.3% (0.13 ± 0.12%), between species ranged from 1.6 to 18.5% (12.8 ± 3.73%), between genera ranged from 13 to 18.6%, and the average in the Accipitridae Family was 11.8%. Therefore, it could form clusters in each species cohesively and clearly separated between the taxa analyzed.","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"82 4 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"BARKODING DNA BURUNG ELANG (FAMILI ACCIPITRIDAE) DI INDONESIA\",\"authors\":\"M. Zein\",\"doi\":\"10.14203/BERITABIOLOGI.V17I2.3108\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The cytochrome c oxidase subunit I (COI) gene is a reprensentative of all the protein-coding genes of the mitochondrial DNA genome that has been widely used as an animal species identification tool. In this study, 86 sequences of DNA barcodes of members of the family Accipitridae in Indonesia including Nisaetus bartelsi, Nisaetus cirrhatus, Haliaeetus leucogaster, Spilornis cheela, Haliastur indus, and 11 sequences from Genbank were examined. Each species was confirmed through the Basic Local Alignment Search Tool (BLAST). The construction of phylogeny trees based on COI gene sequences was performed by the Neighbors-joining method where the calculation of the genetic distance matrix with the Kimura 2-parameter model was implemented in pairwise distance calculation in the Mega version 6.05 programe. The results of the analysis showed that the divergence within species ranged from 0 to 0.3% (0.13 ± 0.12%), between species ranged from 1.6 to 18.5% (12.8 ± 3.73%), between genera ranged from 13 to 18.6%, and the average in the Accipitridae Family was 11.8%. Therefore, it could form clusters in each species cohesively and clearly separated between the taxa analyzed.\",\"PeriodicalId\":280065,\"journal\":{\"name\":\"BERITA BIOLOGI\",\"volume\":\"82 4 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BERITA BIOLOGI\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14203/BERITABIOLOGI.V17I2.3108\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BERITA BIOLOGI","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14203/BERITABIOLOGI.V17I2.3108","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
摘要
细胞色素c氧化酶亚基I (cytochrome c oxidase subunit I, COI)基因是线粒体DNA基因组中所有蛋白质编码基因的代表，已被广泛用作动物物种鉴定工具。本研究对印度尼西亚棘猴科成员(Nisaetus bartelsi、Nisaetus cirrhatus、halieetus leucogaster、Spilornis cheela、Haliastur indus)的86条DNA条形码序列和Genbank中的11条序列进行了分析。每个物种通过BLAST (Basic Local Alignment Search Tool)进行了确认。基于COI基因序列的系统发育树构建采用Neighbors-joining法，遗传距离矩阵的计算采用Kimura 2参数模型，在Mega version 6.05程序中进行两两距离计算。结果表明:种内差异为0 ~ 0.3%(0.13±0.12%)，种间差异为1.6 ~ 18.5%(12.8±3.73%)，属间差异为13 ~ 18.6%，伊蚊科平均差异为11.8%。因此，它可以在每个物种中形成紧密的集群，并在所分析的分类群之间明确区分。
BARKODING DNA BURUNG ELANG (FAMILI ACCIPITRIDAE) DI INDONESIA
The cytochrome c oxidase subunit I (COI) gene is a reprensentative of all the protein-coding genes of the mitochondrial DNA genome that has been widely used as an animal species identification tool. In this study, 86 sequences of DNA barcodes of members of the family Accipitridae in Indonesia including Nisaetus bartelsi, Nisaetus cirrhatus, Haliaeetus leucogaster, Spilornis cheela, Haliastur indus, and 11 sequences from Genbank were examined. Each species was confirmed through the Basic Local Alignment Search Tool (BLAST). The construction of phylogeny trees based on COI gene sequences was performed by the Neighbors-joining method where the calculation of the genetic distance matrix with the Kimura 2-parameter model was implemented in pairwise distance calculation in the Mega version 6.05 programe. The results of the analysis showed that the divergence within species ranged from 0 to 0.3% (0.13 ± 0.12%), between species ranged from 1.6 to 18.5% (12.8 ± 3.73%), between genera ranged from 13 to 18.6%, and the average in the Accipitridae Family was 11.8%. Therefore, it could form clusters in each species cohesively and clearly separated between the taxa analyzed.
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