P. V. Nguyễn, H. Mai, Tuyen T. T. Doan, Thanh T. L. Bien
{"title":"淡紫色紫毛霉11BB株防治根结线虫的潜力评价","authors":"P. V. Nguyễn, H. Mai, Tuyen T. T. Doan, Thanh T. L. Bien","doi":"10.52997/jad.1.01.2022","DOIUrl":null,"url":null,"abstract":"Parasitic fungi are considered effective biological agents to control plant -parasitic nematodes. Based on morphology and ITS sequence, seven Purpureocillium lilacinum strains were isolated from 144 soil samples collected from Chau Duc district, Ba Ria - Vung Tau province, and Dinh Quan district, Dong Nai province. After 96 h of culture in medium supplemented with casein, chitin, and tween 20, seven strains showed protease, chitinase, and lipase activity. In laboratory conditions, both 11BB and 11SN strains parasitzed 31 – 34% eggs and 58 – 62% eggmass, respectively. In greenhouse conditions, the 11BB strain decreased 79.0 - 80.3% of juveniles (J2s) and 77.4 - 79.7% of egg numbers on tomato plants as compared with the control. Results showed that the 11BB fungal strain could be used in control of root - knot nematodes.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"86 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of the control potential to root - knot nematode Meloidogyne incognita of Purpureocillium lilacinum 11BB strain\",\"authors\":\"P. V. Nguyễn, H. Mai, Tuyen T. T. Doan, Thanh T. L. Bien\",\"doi\":\"10.52997/jad.1.01.2022\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Parasitic fungi are considered effective biological agents to control plant -parasitic nematodes. Based on morphology and ITS sequence, seven Purpureocillium lilacinum strains were isolated from 144 soil samples collected from Chau Duc district, Ba Ria - Vung Tau province, and Dinh Quan district, Dong Nai province. After 96 h of culture in medium supplemented with casein, chitin, and tween 20, seven strains showed protease, chitinase, and lipase activity. In laboratory conditions, both 11BB and 11SN strains parasitzed 31 – 34% eggs and 58 – 62% eggmass, respectively. In greenhouse conditions, the 11BB strain decreased 79.0 - 80.3% of juveniles (J2s) and 77.4 - 79.7% of egg numbers on tomato plants as compared with the control. Results showed that the 11BB fungal strain could be used in control of root - knot nematodes.\",\"PeriodicalId\":250563,\"journal\":{\"name\":\"The Journal of Agriculture and Development\",\"volume\":\"86 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-02-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of Agriculture and Development\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.52997/jad.1.01.2022\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Agriculture and Development","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.52997/jad.1.01.2022","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of the control potential to root - knot nematode Meloidogyne incognita of Purpureocillium lilacinum 11BB strain
Parasitic fungi are considered effective biological agents to control plant -parasitic nematodes. Based on morphology and ITS sequence, seven Purpureocillium lilacinum strains were isolated from 144 soil samples collected from Chau Duc district, Ba Ria - Vung Tau province, and Dinh Quan district, Dong Nai province. After 96 h of culture in medium supplemented with casein, chitin, and tween 20, seven strains showed protease, chitinase, and lipase activity. In laboratory conditions, both 11BB and 11SN strains parasitzed 31 – 34% eggs and 58 – 62% eggmass, respectively. In greenhouse conditions, the 11BB strain decreased 79.0 - 80.3% of juveniles (J2s) and 77.4 - 79.7% of egg numbers on tomato plants as compared with the control. Results showed that the 11BB fungal strain could be used in control of root - knot nematodes.