RFLPs Analysis of High pI α-amylase Multigene Families, in Barley (Hordeum vulgare L.)

Analysis of restriction fragment length polymorphisms (RFLPs) revealed that there was at least 3 types of high pI α-amylase multigene families in barley. It was estimated that the isozyme banding patterns detected by the isoelectric-focusing (IEF) method were not directly determined by these 3 types of structural genes, but affected by a regulatory gene (or part). It was assumed that there was a linkage between the structural genes and the regulatory gene, but new combinations of the structural gnes and the regulatory gene could be produced by genetic recombination. The bred lines with the new isozyme banding patterns may have resulted from the new combinations of the genes.