研究突变对花生某些基因型的影响

Samah A. Sabry, Amal M. Abd-El Mageed
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摘要

花生作物具有巨大的经济价值,但其特殊的生长特性给遗传改良带来了一定的困难。本研究旨在通过对4个花生品种的种子进行氮化钠等诱变物质浓度处理,使其遗传变异和改良。实验在伊斯梅利亚研究站实验农场进行;农业研究中心油料作物科。以4个花生品种(吉萨6号、Var.112、Var.57和Var.381)为试验材料,用3种不同浓度的叠氮化钠(NaN 3)处理。对照(0.00),T1(0.01)和T2 (0.03) % W/V诱导突变,随机完全区组设计(RCBD), 3个重复。4个品种的总平均值在M1代高于M2代。T1和T2突变植株的茎高均高于对照植株,低剂量诱变剂处理下M2代单株分枝数增幅最大,而低剂量诱变剂处理下两代种子数和百粒重均低于对照。表型变异系数(P.C.V. %)和基因型变异系数(G.C.V. %)在M1代均大于M2代。在SPAD、茎高、枝/株数、荚果数和荚果产量(Ardab / Fadden)方面,GCV均低于PCV。籽粒重/株、百粒重、籽粒产量(种子数/株、籽粒重/株、百粒重)和含油率的GCV与PCV基本相等。M1代和M2代的所有性状遗传力值均超过65%,M2代的T2突变株遗传力得分高于M1代。大部分性状的遗传选择增益(GS)值在82% ~ 99.88%之间,M1代和M2代的遗传选择增益(GS)值分别在0.7 ~ 105.8和2.5 ~ 108.7之间。0.01%和0.03%叠氮化钠对花生数量性状的诱导突变有重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studying the Effect of Mutations on some genotypes of Peanut
: Peanut crop has a great economic value and it has special growth nature caused some difficulties in genetic improvement. This study aims to carry some genetic variations and improvement through treating seeds of four varieties of peanut with concentrations of mutagenic substance like sodium azid. An experiment was conducted at the Experimental Farm, Ismailia Research Station; Oil Crops Section- Agricultural Research Center (A.R.C). Four varieties of peanut (Giza 6, Var.112, Var.57, and Var.381) were treated with three different concentrations of sodium azide (NaN 3 ); 0.00 control (0.00), T1 (0.01) and T2 (0.03) % W/V for inducing mutation and planted in a randomized complete block design (RCBD) with three replications. The general mean of all 4 varieties was higher in the M1 than those in the M2 generation. T1 and T2 mutated plants displayed a high value of the stem height compared to control plants for all 4 varieties, The largest increase in branches per plant was observed in the M2 generation at a low dose of mutagen while the seeds number and 100 seeds weight were less than control at the low dose of mutagen in both generations. The values of phenotypic coefficient of variability (P.C.V. %) and genotypic coefficient of variability (G.C.V. %) were greater in the M1 generation than those in the M2 generation. GCV was less than PCV in SPAD, stem height, number of branches/plant, number of pods/plant and pod yield (Ardab / Fadden ) . While GCV was more or less equal to PCV for pod weight/plant, 100 pod weight, and seed yield (seeds number/plant, seed weight/plant, and 100 seed weight) as well as oil percentage. Heritability values exceeded 65% for all traits examined in both the M1 and M2 generations, T2 mutant plants in the M2 generation had higher heritability scores than those in the M1 generation. They scored values between 82% and 99.88% for most of the traits investigated and all studied traits recorded different values of genetic gain by selection (GS), ranging from 0.7 to 105.8 and from 2.5 to 108.7 in M1 and M2 generations, respectively. 0.01% and 0.03% of sodium azide played an important role in inducing mutation to improve the quantitative characters of selected peanut varieties.
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